Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown

Daqian Sun, Margherita Melegari, Sunandini Sridhar, Charles E. Rogler, Liang Zhu

Research output: Contribution to journalArticlepeer-review

117 Scopus citations

Abstract

A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalBioTechniques
Volume41
Issue number1
DOIs
StatePublished - Jul 2006

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry, Genetics and Molecular Biology

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