Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown

Daqian Sun, Margherita Melegari, Sunandini Sridhar, Charles E. Rogler, Liang Zhu

Research output: Contribution to journalArticle

109 Citations (Scopus)

Abstract

A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalBioTechniques
Volume41
Issue number1
DOIs
StatePublished - Jul 2006

Fingerprint

Gene Knockdown Techniques
MicroRNAs
Genes
Nucleotides
RNA

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown. / Sun, Daqian; Melegari, Margherita; Sridhar, Sunandini; Rogler, Charles E.; Zhu, Liang.

In: BioTechniques, Vol. 41, No. 1, 07.2006, p. 59-63.

Research output: Contribution to journalArticle

Sun, Daqian ; Melegari, Margherita ; Sridhar, Sunandini ; Rogler, Charles E. ; Zhu, Liang. / Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown. In: BioTechniques. 2006 ; Vol. 41, No. 1. pp. 59-63.
@article{75e0886fb932483db60fd22773a321be,
title = "Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown",
abstract = "A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.",
author = "Daqian Sun and Margherita Melegari and Sunandini Sridhar and Rogler, {Charles E.} and Liang Zhu",
year = "2006",
month = "7",
doi = "10.2144/000112203",
language = "English (US)",
volume = "41",
pages = "59--63",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "1",

}

TY - JOUR

T1 - Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown

AU - Sun, Daqian

AU - Melegari, Margherita

AU - Sridhar, Sunandini

AU - Rogler, Charles E.

AU - Zhu, Liang

PY - 2006/7

Y1 - 2006/7

N2 - A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.

AB - A number of natural microRNA (miRNA) hairpins have been found in clusters of multiple identical or different copies, suggesting that effects of miRNAs can be enhanced and multiple genes can be regulated together by encoding multiple miRNA hairpins in a single transcript. Here, we report a simple and effective artificial multi-hairpin method that stimulates production of mature 22-nucleotide small RNAs from modified miRNA hairpins, improves gene knockdown over single-hairpin constructs, and provides linked multi-gene knockdowns.

UR - http://www.scopus.com/inward/record.url?scp=33747603277&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747603277&partnerID=8YFLogxK

U2 - 10.2144/000112203

DO - 10.2144/000112203

M3 - Article

VL - 41

SP - 59

EP - 63

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 1

ER -