Abstract
Motivation: Bead arrays are becoming a popular platform for high-throughput expression arrays. However, the number of the beads targeting a transcript and the variation of their intensities differ from sample to sample in these arrays. This property results in different accuracy of expression intensities of a transcript across arrays. Results: We provide evidence, with publicly available spike-in data, that the false discovery rate of differential expression is reduced by modeling bead-level variability with a multi-level mixed effects model. We compare the performance of our proposed model to existing analysis methods for bead arrays: the unweighted t-test and other weighted methods. Additionally, we provide theoretical insights into when the multi-level mixed effects model outperforms other methods. Finally, we provide a software program for differential expression analysis using the multi-level mixed effects model that analyzes tens of thousands of genes efficiently.
Original language | English (US) |
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Article number | btq708 |
Pages (from-to) | 633-640 |
Number of pages | 8 |
Journal | Bioinformatics |
Volume | 27 |
Issue number | 5 |
DOIs | |
State | Published - Mar 2011 |
ASJC Scopus subject areas
- Statistics and Probability
- Biochemistry
- Molecular Biology
- Computer Science Applications
- Computational Theory and Mathematics
- Computational Mathematics