Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae

Shilpi Chandra; James Gray; William B. Kiosses; Archana Khurana; Kaori Hitomi; Catherine M. Crosby; Ashu Chawla; Zheng Fu; Meng Zhao; Natacha Veerapen; Stewart K. Richardson; Steven A. Porcelli; Gurdyal Besra; Amy R. Howell; Sonia Sharma; Bjoern Peters; Mitchell Kronenberg

doi:10.1038/s41467-018-06646-8

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae

Shilpi Chandra, James Gray, William B. Kiosses, Archana Khurana, Kaori Hitomi, Catherine M. Crosby, Ashu Chawla, Zheng Fu, Meng Zhao, Natacha Veerapen, Stewart K. Richardson, Steven A. Porcelli, Gurdyal Besra, Amy R. Howell, Sonia Sharma, Bjoern Peters, Mitchell Kronenberg

Microbiology & Immunology

Research output: Contribution to journal › Article

1 Scopus citations

Abstract

Invariant natural killer T cells (iNKT cells) are activated by lipid antigens presented by CD1d, but the pathway leading to lipid antigen presentation remains incompletely characterized. Here we show a whole-genome siRNA screen to elucidate the CD1d presentation pathway. A majority of gene knockdowns that diminish antigen presentation reduced formation of glycolipid-CD1d complexes on the cell surface, including members of the HOPS and ESCRT complexes, genes affecting cytoskeletal rearrangement, and ABC family transporters. We validated the role in vivo for the multidrug resistance protein 1 (Mrp1) in CD1d antigen presentation. Mrp1 deficiency reduces surface clustering of CD1d, which decreased iNKT cell activation. Infected Mrp1 knockout mice show decreased iNKT cell responses to antigens from Streptococcus pneumoniae and were associated with increased mortality. Our results highlight the unique cellular events involved in lipid antigen presentation and show how modification of this pathway can lead to lethal infection.

Original language	English (US)
Article number	4279
Journal	Nature communications
Volume	9
Issue number	1
DOIs	https://doi.org/10.1038/s41467-018-06646-8
State	Published - Dec 1 2018

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ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

Cite this

Chandra, S., Gray, J., Kiosses, W. B., Khurana, A., Hitomi, K., Crosby, C. M., Chawla, A., Fu, Z., Zhao, M., Veerapen, N., Richardson, S. K., Porcelli, S. A., Besra, G., Howell, A. R., Sharma, S., Peters, B., & Kronenberg, M. (2018). Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nature communications, 9(1), [4279]. https://doi.org/10.1038/s41467-018-06646-8

Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. / Chandra, Shilpi; Gray, James; Kiosses, William B.; Khurana, Archana; Hitomi, Kaori; Crosby, Catherine M.; Chawla, Ashu; Fu, Zheng; Zhao, Meng; Veerapen, Natacha; Richardson, Stewart K.; Porcelli, Steven A.; Besra, Gurdyal; Howell, Amy R.; Sharma, Sonia; Peters, Bjoern; Kronenberg, Mitchell.

In: Nature communications, Vol. 9, No. 1, 4279, 01.12.2018.

Research output: Contribution to journal › Article

Chandra, S, Gray, J, Kiosses, WB, Khurana, A, Hitomi, K, Crosby, CM, Chawla, A, Fu, Z, Zhao, M, Veerapen, N, Richardson, SK, Porcelli, SA, Besra, G, Howell, AR, Sharma, S, Peters, B & Kronenberg, M 2018, 'Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae', Nature communications, vol. 9, no. 1, 4279. https://doi.org/10.1038/s41467-018-06646-8

Chandra, Shilpi ; Gray, James ; Kiosses, William B. ; Khurana, Archana ; Hitomi, Kaori ; Crosby, Catherine M. ; Chawla, Ashu ; Fu, Zheng ; Zhao, Meng ; Veerapen, Natacha ; Richardson, Stewart K. ; Porcelli, Steven A. ; Besra, Gurdyal ; Howell, Amy R. ; Sharma, Sonia ; Peters, Bjoern ; Kronenberg, Mitchell. / Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. In: Nature communications. 2018 ; Vol. 9, No. 1.

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10.1038/s41467-018-06646-8