mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

P. Eline Slagboom, Wiljo J.F. de Leeuw, Jan Vijg

Research output: Contribution to journalArticle

7 Scopus citations


We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

Original languageEnglish (US)
Pages (from-to)128-130
Number of pages3
JournalFEBS Letters
Issue number1
StatePublished - Aug 20 1990



  • Aging
  • DNA methylation
  • Tyrosine aminotransferase
  • mRNA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this