mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats

P. Eline Slagboom, Wiljo J F de Leeuw, Jan Vijg

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have examined the mRNA levels and methylation patterns of the liver-specific tyrosine aminotransferase (TAT) gene in inbred female rats aged 6, 24 and 36 months. Northern hybridization analysis of total RNA showed a 65% decrease in the steady state transcript level of TAT in the liver of 24- and 36-month old rats as compared to 6-month old animals. The TAT gene as studied by Southern hybridization analysis using the isoschizomers Hpa II and Msp I was found to be hypomethylated in the liver as compared to spleen and brain at six CpG sites within the gene. Methylation at these sites remained unchanged during aging.

Original languageEnglish (US)
Pages (from-to)128-130
Number of pages3
JournalFEBS Letters
Volume269
Issue number1
DOIs
StatePublished - Aug 20 1990
Externally publishedYes

Fingerprint

Tyrosine Transaminase
Methylation
Liver
Rats
Genes
Aging of materials
Messenger RNA
Brain
Animals
Spleen
RNA

Keywords

  • Aging
  • DNA methylation
  • mRNA
  • Tyrosine aminotransferase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats. / Slagboom, P. Eline; de Leeuw, Wiljo J F; Vijg, Jan.

In: FEBS Letters, Vol. 269, No. 1, 20.08.1990, p. 128-130.

Research output: Contribution to journalArticle

Slagboom, P. Eline ; de Leeuw, Wiljo J F ; Vijg, Jan. / mRNA levels and methylation patterns of the tyrosine aminotransferase gene in aging inbred rats. In: FEBS Letters. 1990 ; Vol. 269, No. 1. pp. 128-130.
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