MRG1 Expression in Fibroblasts Is Regulated by Sp1/Sp3 and an Ets Transcription Factor

Baoguang Han, Naili Liu, Xiaoming Yang, Hui (Herb) Sun, Yu Chung Yang

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

MRG1 (melanocyte-specific gene 1 (MSG1)-related gene), a ubiquitously expressed transcription factor that interacts with p300/CBP, TATA-binding protein and Lhx2, is the founding member of a new family of transcription factors. Initial characterization of this newly discovered transcription factor has underscored its potential involvement in many important cellular processes through transcriptional modulation. We previously demonstrated that MRG1 can be induced by various biological stimuli (Sun, H. B., Zhu, Y. X., Yin, T., Sledge, G., and Yang, Y. C. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 13555-13560). As a first step in understanding its role in different biological processes, we investigated mechanisms that regulate transcription of the mouse MRG1 gene in fibroblasts. Transient transfection of Rat1 fibroblast cells with sequential 5′-deletions of mouse MRG1 promoter-luciferase fusion constructs indicated that the -104 to +121 region contains the full promoter activity. Deletion and site-directed mutations within this region revealed that the Ets-1 site at -97 to -94 and the Sp1 site at -51 to -46 are critical for MRG1 expression in fibroblasts. Gel mobility shift and super-shift assays performed with Rat1 nuclear extracts identified nucleoprotein complexes binding to the Ets-1 site and the Sp1 site. In Drosophila SL2 cells, which lack the Sp and Ets family of transcription factors, expression of Sp1, Sp3, and Ets-1 or Elf-1 functionally stimulated MRG1 promoter activity in a synergistic manner. These results suggest that multiple transcription factors acting in synergy are responsible for MRG1 expression and the responsiveness of cells to different biological stimuli.

Original languageEnglish (US)
Pages (from-to)7937-7942
Number of pages6
JournalJournal of Biological Chemistry
Volume276
Issue number11
DOIs
StatePublished - Mar 16 2001
Externally publishedYes

Fingerprint

Sp3 Transcription Factor
Proto-Oncogene Proteins c-ets
Fibroblasts
Transcription Factors
Genes
TATA-Box Binding Protein
Biological Phenomena
Nucleoproteins
Melanocytes
Luciferases
Drosophila
Transfection
Transcription
Gels
Assays
Fusion reactions
Mutation
Cells
Modulation

ASJC Scopus subject areas

  • Biochemistry

Cite this

MRG1 Expression in Fibroblasts Is Regulated by Sp1/Sp3 and an Ets Transcription Factor. / Han, Baoguang; Liu, Naili; Yang, Xiaoming; Sun, Hui (Herb); Yang, Yu Chung.

In: Journal of Biological Chemistry, Vol. 276, No. 11, 16.03.2001, p. 7937-7942.

Research output: Contribution to journalArticle

Han, Baoguang ; Liu, Naili ; Yang, Xiaoming ; Sun, Hui (Herb) ; Yang, Yu Chung. / MRG1 Expression in Fibroblasts Is Regulated by Sp1/Sp3 and an Ets Transcription Factor. In: Journal of Biological Chemistry. 2001 ; Vol. 276, No. 11. pp. 7937-7942.
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