TY - JOUR
T1 - Mouse T cell antigen receptor
T2 - Structure and organization of constant and joining gene segments encoding the β polypeptide
AU - Malissen, Marie
AU - Minard, Karyl
AU - Mjolsness, Shelley
AU - Kronenberg, Mitchell
AU - Goverman, Joan
AU - Hunkapiller, Tim
AU - Prystowsky, Michael B.
AU - Yoshikai, Yasunobu
AU - Fitch, Frank
AU - Mak, Tak W.
AU - Hood, Leroy
N1 - Funding Information:
The authors thank Elizabeth Gibb for her help during the construction of the cosmid library, Dr. Suzanna Horvath for the synthetic oligonucleotides, Gerald Siu and Drs. Martha Zuniga, Joan Kobori, and Richard Barth for critical reading of the manuscript. We also want to thank Debbie Maloney for technical assistance and Bertha Jones, Bernita Larsh, and Connie Katz for the preparation of the manuscript, M. M. is supported by the Centre National de la Recherche Scientifique (France). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
PY - 1984/7
Y1 - 1984/7
N2 - The germ-line joining (J) gene segments and constant (C) genes encoding the β chain of the mouse T cell antigen receptor have been isolated on a single cosmid clone. There are two constant genes, Cβ1 and Cβ2, each associated with a cluster of Jβ gene segments. The nucleotide sequences of the Cβ2 gene and of the Jβ2 cluster gene segments have been determined. The coding sequence of the Cβ2 gene is very similar to the sequence of a cDNA clone encoded by the Cβ1 gene. The Cβ2 gene has four exons; exon-intron structure does not obviously correspond to the functional domains of the protein. The Jβ2 gene segment cluster contains six functional J gene segments. We have isolated specific probes for the Cβ1, Cβ2, Jβ1, and Jβ2 regions to examine DNA rearrangements in T lymphocytes. DNA rearrangements can occur in both Jβ gene segment clusters, and both Cβ genes appear functional.
AB - The germ-line joining (J) gene segments and constant (C) genes encoding the β chain of the mouse T cell antigen receptor have been isolated on a single cosmid clone. There are two constant genes, Cβ1 and Cβ2, each associated with a cluster of Jβ gene segments. The nucleotide sequences of the Cβ2 gene and of the Jβ2 cluster gene segments have been determined. The coding sequence of the Cβ2 gene is very similar to the sequence of a cDNA clone encoded by the Cβ1 gene. The Cβ2 gene has four exons; exon-intron structure does not obviously correspond to the functional domains of the protein. The Jβ2 gene segment cluster contains six functional J gene segments. We have isolated specific probes for the Cβ1, Cβ2, Jβ1, and Jβ2 regions to examine DNA rearrangements in T lymphocytes. DNA rearrangements can occur in both Jβ gene segment clusters, and both Cβ genes appear functional.
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U2 - 10.1016/0092-8674(84)90444-6
DO - 10.1016/0092-8674(84)90444-6
M3 - Article
C2 - 6611207
AN - SCOPUS:0021669351
SN - 0092-8674
VL - 37
SP - 1101
EP - 1110
JO - Cell
JF - Cell
IS - 3
ER -