Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide

Marie Malissen, Karyl Minard, Shelley Mjolsness, Mitchell Kronenberg, Joan Goverman, Tim Hunkapiller, Michael B. Prystowsky, Yasunobu Yoshikai, Frank Fitch, Tak W. Mak, Leroy Hood

Research output: Contribution to journalArticle

299 Citations (Scopus)

Abstract

The germ-line joining (J) gene segments and constant (C) genes encoding the β chain of the mouse T cell antigen receptor have been isolated on a single cosmid clone. There are two constant genes, Cβ1 and Cβ2, each associated with a cluster of Jβ gene segments. The nucleotide sequences of the Cβ2 gene and of the Jβ2 cluster gene segments have been determined. The coding sequence of the Cβ2 gene is very similar to the sequence of a cDNA clone encoded by the Cβ1 gene. The Cβ2 gene has four exons; exon-intron structure does not obviously correspond to the functional domains of the protein. The Jβ2 gene segment cluster contains six functional J gene segments. We have isolated specific probes for the Cβ1, Cβ2, Jβ1, and Jβ2 regions to examine DNA rearrangements in T lymphocytes. DNA rearrangements can occur in both Jβ gene segment clusters, and both Cβ genes appear functional.

Original languageEnglish (US)
Pages (from-to)1101-1110
Number of pages10
JournalCell
Volume37
Issue number3
DOIs
StatePublished - 1984
Externally publishedYes

Fingerprint

Gene encoding
T-Cell Antigen Receptor
Joining
Genes
Peptides
Multigene Family
Gene Rearrangement
Exons
Clone Cells
Cosmids
Germ Cells
Introns
T-cells
DNA
Complementary DNA
T-Lymphocytes
Nucleotides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Malissen, M., Minard, K., Mjolsness, S., Kronenberg, M., Goverman, J., Hunkapiller, T., ... Hood, L. (1984). Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide. Cell, 37(3), 1101-1110. https://doi.org/10.1016/0092-8674(84)90444-6

Mouse T cell antigen receptor : Structure and organization of constant and joining gene segments encoding the β polypeptide. / Malissen, Marie; Minard, Karyl; Mjolsness, Shelley; Kronenberg, Mitchell; Goverman, Joan; Hunkapiller, Tim; Prystowsky, Michael B.; Yoshikai, Yasunobu; Fitch, Frank; Mak, Tak W.; Hood, Leroy.

In: Cell, Vol. 37, No. 3, 1984, p. 1101-1110.

Research output: Contribution to journalArticle

Malissen, M, Minard, K, Mjolsness, S, Kronenberg, M, Goverman, J, Hunkapiller, T, Prystowsky, MB, Yoshikai, Y, Fitch, F, Mak, TW & Hood, L 1984, 'Mouse T cell antigen receptor: Structure and organization of constant and joining gene segments encoding the β polypeptide', Cell, vol. 37, no. 3, pp. 1101-1110. https://doi.org/10.1016/0092-8674(84)90444-6
Malissen, Marie ; Minard, Karyl ; Mjolsness, Shelley ; Kronenberg, Mitchell ; Goverman, Joan ; Hunkapiller, Tim ; Prystowsky, Michael B. ; Yoshikai, Yasunobu ; Fitch, Frank ; Mak, Tak W. ; Hood, Leroy. / Mouse T cell antigen receptor : Structure and organization of constant and joining gene segments encoding the β polypeptide. In: Cell. 1984 ; Vol. 37, No. 3. pp. 1101-1110.
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