Mouse mesangial cells produce colony-stimulating factor-1 (CSF-1) and express the CSF-1 receptor

T. Mori, A. Bartocci, J. Satriano, A. Zuckerman, R. Stanley, A. Santiago, D. Schlondorff

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13 Scopus citations


CSF-1 stimulates the survival, proliferation, and differentiation of mononuclear phagocytes and may also play a role in placental development. The expression of CSF-1 and the CSF-1 receptor (CSF-1R) and their regulation were examined in cultures of mouse mesangial cells (MC). The concentration of CSF-1 in the medium of cultured MC increased linearly with time over 24 h. IFN-γ stimulated and dibutyryl cyclic AMP inhibited CSF-1 production in a dose-dependent manner. MC expression of CSF-1 mRNA was shown by Northern blot analysis, and CSF-1 mRNA levels were increased within 4 h of IFN-γ addition and inhibited within 4 h of dibutyryl cyclic AMP addition. Indirect immunofluorescence indicated that 90% of the untreated cultured MC expressed CSF-1. In addition, CSF-1R expression by MC was demonstrated by immunofluorescence with anti-receptor antibody, specific binding of [125I]CSF-1, and expression of the CSF-1R mRNA by Northern blot analysis. Thus, mouse MC, specialized pericytes of non-bone marrow origin, not only produce CSF-1 but also express receptors for CSF-1. The effects of CSF-1 on MC may be important in the control of immune function in the glomerulus.

Original languageEnglish (US)
Pages (from-to)4697-4702
Number of pages6
JournalJournal of Immunology
Issue number12
StatePublished - 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


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