Mother's milk-induced hsp70 expression preserves intestinal epithelial barrier function in an immature rat pup model

Jennifer L. Liedel, Yuee Guo, Yueyue Yu, Sheng Ru Shiou, Sangzi Chen, Elaine O. Petrof, Shien Hu, Mark W. Musch, Erika C. Claud

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Preterm infants face many challenges in transitioning from the in utero to extrauterine environment while still immature. Failure of the preterm gut to successfully mature to accommodate bacteria and food substrate leads to significant morbidity such as neonatal necrotizing enterocolitis. The intestinal epithelial barrier plays a critical role in gut protection. Heat shock protein 70 (Hsp70) is an inducible cytoprotective molecule shown to protect the intestinal epithelium in adult models. To investigate the hypothesis that Hsp70 may be important for early protection of the immature intestine, Hsp70 expression was evaluated in intestine of immature rat pups. Data demonstrate that Hsp70 is induced by exposure to mother's milk. Hsp70 is found in mother's milk, and increased Hsp70 transcription is induced by mother's milk. This Hsp70 colocalizes with the tight junction protein ZO-1. Mother's milk-induced Hsp70 may contribute to maintenance of barrier function in the face of oxidant stress. Further understanding of the means by which mother's milk increases Hsp70 in the ileum will allow potential means of strengthening the intestinal barrier in at-risk preterm infants.

Original languageEnglish (US)
Pages (from-to)395-400
Number of pages6
JournalPediatric Research
Volume69
Issue number5 PART 1
DOIs
StatePublished - May 2011
Externally publishedYes

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HSP70 Heat-Shock Proteins
Milk
Mothers
Premature Infants
Intestines
Zonula Occludens-1 Protein
Necrotizing Enterocolitis
Intestinal Mucosa
Ileum
Oxidants
Maintenance
Morbidity
Bacteria
Food

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Liedel, J. L., Guo, Y., Yu, Y., Shiou, S. R., Chen, S., Petrof, E. O., ... Claud, E. C. (2011). Mother's milk-induced hsp70 expression preserves intestinal epithelial barrier function in an immature rat pup model. Pediatric Research, 69(5 PART 1), 395-400. https://doi.org/10.1203/PDR.0b013e3182114ec9

Mother's milk-induced hsp70 expression preserves intestinal epithelial barrier function in an immature rat pup model. / Liedel, Jennifer L.; Guo, Yuee; Yu, Yueyue; Shiou, Sheng Ru; Chen, Sangzi; Petrof, Elaine O.; Hu, Shien; Musch, Mark W.; Claud, Erika C.

In: Pediatric Research, Vol. 69, No. 5 PART 1, 05.2011, p. 395-400.

Research output: Contribution to journalArticle

Liedel, JL, Guo, Y, Yu, Y, Shiou, SR, Chen, S, Petrof, EO, Hu, S, Musch, MW & Claud, EC 2011, 'Mother's milk-induced hsp70 expression preserves intestinal epithelial barrier function in an immature rat pup model', Pediatric Research, vol. 69, no. 5 PART 1, pp. 395-400. https://doi.org/10.1203/PDR.0b013e3182114ec9
Liedel, Jennifer L. ; Guo, Yuee ; Yu, Yueyue ; Shiou, Sheng Ru ; Chen, Sangzi ; Petrof, Elaine O. ; Hu, Shien ; Musch, Mark W. ; Claud, Erika C. / Mother's milk-induced hsp70 expression preserves intestinal epithelial barrier function in an immature rat pup model. In: Pediatric Research. 2011 ; Vol. 69, No. 5 PART 1. pp. 395-400.
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