Morphometric analysis in neurodegenerative disorders

Dejan Milatovic, Thomas J. Montine, Snjezana Zaja-Milatovic, Jennifer L. Madison, Aaron B. Bowman, Michael Aschner

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The study of dendritic length and spine density has become standard in the analysis of neuronal abnormalities, since a considerable number of neurological diseases have their foundation in alterations in these structures. One of the best ways to study possible alterations in neuronal morphometry is the use of Golgi impregnation. Introduced more than a century ago, it is still the standard and state-of-the-art technique for visualization of neuronal architecture. We successfully applied the Golgi method to mouse, rat, monkey, and human brain tissues for studying both the normal and abnormal morphology of neurons. We were able to discover subtle morphological alterations in neuronal dendrites and dendritic spines in different brain areas. Although Golgi preparations can be examined by electronic microscopy, we used light microscopy and reconstruction using Neurolucida software to quantitatively explore the relationship between total dendritic length and spine density in different types of neurons. This unit summarizes the methodology used to quantify neuronal abnormalities and discusses the utility of these techniques in different models of neurodegeneration.

Original languageEnglish (US)
Pages (from-to)12.16.1-12.16.14
JournalCurrent Protocols in Toxicology
Issue numberSUPPL. 43
DOIs
StatePublished - Mar 1 2010
Externally publishedYes

Keywords

  • Dendrites
  • Dendritic spine
  • Golgi impregnation
  • Neurolucida
  • Neuronal morphometry

ASJC Scopus subject areas

  • Toxicology

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    Milatovic, D., Montine, T. J., Zaja-Milatovic, S., Madison, J. L., Bowman, A. B., & Aschner, M. (2010). Morphometric analysis in neurodegenerative disorders. Current Protocols in Toxicology, (SUPPL. 43), 12.16.1-12.16.14. https://doi.org/10.1002/0471140856.tx1216s43