Morphologic changes in the liver of dialyzed rats--preliminary observations.

Katarzyna Wieczorowska-Tobis, Renata Brelinska, Andrzej Breborowicz, Dimitrios G. Oreopoulos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Peritoneal dialysis affects both the quantity and quality of connective tissue in the visceral peritoneum. In the present study, we report the alterations observed in the morphology of the superficial liver lobuli of dialyzed rats. The studies were performed in male Wistar rats weighing 250-350 g. Rats were exposed intraperitoneally to 0.9% NaCl, phosphate-buffered saline (PBS), or commercial dialysis solutions containing 3.86% glucose (Dianeal 3.86%: Baxter Healthcare SA, Castlebar, Ireland; CAPD3 and CAPD3-Balance: Fresenius Medical Care, Bad Homburg, Germany) twice daily for 4-6 weeks. At the end of the study, samples of the liver were taken and stained for light microscopy (hematoxylin and eosin, van Gieson). The results obtained in dialyzed rats were compared with those from the control, non dialyzed animals. In control animals, the surface of the hepatic parenchyma was smooth. In all dialyzed rats, irrespective of the solution used, folding of the surface of the liver parenchyma was found owing to penetration of connective tissue elements between the hepatocytes. In effect, folds of hepatocytes within the liver capsule became detached and isolated from the remaining cells of the lobules. The distinctive feature of that pathologic change was that its severity increased with the thickness of the peritoneum. The change was seen in rats exposed to any of the experimental solutions, and therefore appeared to be attributable to a non-specific reaction caused by exposure of the peritoneum to dialysis fluid and not to specific components of the fluid. The observed alterations in morphology seem to suggest disturbed function within the affected lobules. Further studies are necessary to confirm this hypothesis.

Original languageEnglish (US)
Pages (from-to)20-23
Number of pages4
JournalAdvances in peritoneal dialysis. Conference on Peritoneal Dialysis
Volume19
StatePublished - 2003
Externally publishedYes

Fingerprint

Peritoneum
Liver
Connective Tissue
Hepatocytes
Dialysis Solutions
Peritoneal Dialysis
Hematoxylin
Eosine Yellowish-(YS)
Ireland
Capsules
Germany
Wistar Rats
Dialysis
Microscopy
Phosphates
Delivery of Health Care
Light
Glucose

Cite this

Wieczorowska-Tobis, K., Brelinska, R., Breborowicz, A., & Oreopoulos, D. G. (2003). Morphologic changes in the liver of dialyzed rats--preliminary observations. Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, 19, 20-23.

Morphologic changes in the liver of dialyzed rats--preliminary observations. / Wieczorowska-Tobis, Katarzyna; Brelinska, Renata; Breborowicz, Andrzej; Oreopoulos, Dimitrios G.

In: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, Vol. 19, 2003, p. 20-23.

Research output: Contribution to journalArticle

Wieczorowska-Tobis, K, Brelinska, R, Breborowicz, A & Oreopoulos, DG 2003, 'Morphologic changes in the liver of dialyzed rats--preliminary observations.', Advances in peritoneal dialysis. Conference on Peritoneal Dialysis, vol. 19, pp. 20-23.
Wieczorowska-Tobis, Katarzyna ; Brelinska, Renata ; Breborowicz, Andrzej ; Oreopoulos, Dimitrios G. / Morphologic changes in the liver of dialyzed rats--preliminary observations. In: Advances in peritoneal dialysis. Conference on Peritoneal Dialysis. 2003 ; Vol. 19. pp. 20-23.
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