TY - JOUR
T1 - Monoclonal antibody based ELISAs for cryptococcal polysaccharide
AU - Casadevall, Arturo
AU - Mukherjee, Jean
AU - Scharff, Matthew D.
N1 - Funding Information:
The fungus Cryptococcus neoformans causes life-threatening meningoencephalitis in up to 10% of patients with the acquired immunodeficiency syndrome (Zuger et al., 1986). This fungus is Albert Einstein College of Medicine, 1300 Morris Park Ave., Chanin Room 330, Bronx, NY 10461, USA. * This work was supported by N1H grants CA09173 and CA39838. A.C. is supported by a Pfizer Postdoctoral Fellowship. J.M. is supported by NIH training grant 2T32C809173-16. M.D.S. is supported in part by the Harry Eagle Chair in Cancer Research from the Women's Division of the Albert Einstein College of Medicine.
PY - 1992/9/18
Y1 - 1992/9/18
N2 - Mouse monoclonal antibody (MAb)-based enzyme-linked immunosorbent assays (ELISAs) have been developed to detect Cryptococcus neoformans capsular polysaccharides from the four serotypes A, B, C and D. The ELISAs avoid the problem of unreliable polysaccharide binding to polystyrene plates by using MAbs to capture and immobilize the polysaccharide antigen. The presence of polysaccharide is detected using MAbs of a different isotype from that of the capture MAb. The capturing MAbs are themselves immobilized on the plates using commercial goal anti-mouse polyclonal sera. The MAbs bind to the glucuronoxylomannan component of cryptococcal polysaccharide. The ELISAs can be used to measure the concentration of polysaccharide in biological fluids and are potentially useful tools for basic research and clinical studies.
AB - Mouse monoclonal antibody (MAb)-based enzyme-linked immunosorbent assays (ELISAs) have been developed to detect Cryptococcus neoformans capsular polysaccharides from the four serotypes A, B, C and D. The ELISAs avoid the problem of unreliable polysaccharide binding to polystyrene plates by using MAbs to capture and immobilize the polysaccharide antigen. The presence of polysaccharide is detected using MAbs of a different isotype from that of the capture MAb. The capturing MAbs are themselves immobilized on the plates using commercial goal anti-mouse polyclonal sera. The MAbs bind to the glucuronoxylomannan component of cryptococcal polysaccharide. The ELISAs can be used to measure the concentration of polysaccharide in biological fluids and are potentially useful tools for basic research and clinical studies.
KW - Cryptococcus neoformans
KW - ELISA
KW - Immunoglobulin, mouse
KW - Monoclonal antibody, mouse
KW - Polysaccharide, cryptococcal
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U2 - 10.1016/0022-1759(92)90209-C
DO - 10.1016/0022-1759(92)90209-C
M3 - Article
C2 - 1401941
AN - SCOPUS:0026669252
SN - 0022-1759
VL - 154
SP - 27
EP - 35
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 1
ER -