Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria

Aharona Glatman-Freedman, Jean M. Martin, Paul F. Riska, Barry R. Bloom, Arturo Casadevall

Research output: Contribution to journalArticle

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Abstract

Three monoclonal antibodies (MAbs) were generated from splenocytes of a BALB/c mouse immunized with heat-killed Mycobacterium tuberculosis. All three MAbs bound to surface epitopes of M. tuberculosis as shown by whole-cell enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence, and immunoelectron microscopy. One immunoglobulin M (IgM) MAb bound to lipoarabinomannan, the second IgM MAb bound to mycolyl-arabinogalactan- peptidoglycan complex, and the third MAb, an IgC3, bound to a surface epitope of an uncertain nature. The MAbs demonstrated different cross-reactivity patterns with other mycobacteria. Two of the MAbs were used to develop a modified ELISA spot assay for the detection of mycobacteria.

Original languageEnglish (US)
Pages (from-to)2795-2802
Number of pages8
JournalJournal of Clinical Microbiology
Volume34
Issue number11
StatePublished - Nov 1996

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Surface Antigens
Mycobacterium
Mycobacterium tuberculosis
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Immunoglobulin M
Epitopes
Immunoelectron Microscopy
Indirect Fluorescent Antibody Technique
Fluorescence Microscopy
Hot Temperature

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Monoclonal antibodies to surface antigens of Mycobacterium tuberculosis and their use in a modified enzyme-linked immunosorbent spot assay for detection of mycobacteria. / Glatman-Freedman, Aharona; Martin, Jean M.; Riska, Paul F.; Bloom, Barry R.; Casadevall, Arturo.

In: Journal of Clinical Microbiology, Vol. 34, No. 11, 11.1996, p. 2795-2802.

Research output: Contribution to journalArticle

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