TY - JOUR
T1 - Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51
AU - Al-Sayyed, Ban
AU - Piperdi, Sajida
AU - Yuan, Xinni
AU - Li, Anping
AU - Besra, Gurdyal S.
AU - Jacobs, William R.
AU - Casadevall, Arturo
AU - Glatman-Freedman, Aharona
N1 - Funding Information:
Funding : This work was supported by a grant from the center for AIDS research at the Albert Einstein College of Medicine to A.G-F and in part by NIH grants AI001691 and AI053192 to A.G-F.
PY - 2007/11
Y1 - 2007/11
N2 - Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
AB - Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
KW - MPT51
KW - Monoclonal antibodies
KW - Mycobacterium tuberculosis
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U2 - 10.1016/j.tube.2007.07.005
DO - 10.1016/j.tube.2007.07.005
M3 - Article
C2 - 17881291
AN - SCOPUS:35348872454
VL - 87
SP - 489
EP - 497
JO - Bulletin of the International Union Against Tuberculosis and Lung Disease
JF - Bulletin of the International Union Against Tuberculosis and Lung Disease
SN - 1472-9792
IS - 6
ER -
