Abstract
Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 489-497 |
| Number of pages | 9 |
| Journal | Tuberculosis |
| Volume | 87 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1 2007 |
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Keywords
- Monoclonal antibodies
- MPT51
- Mycobacterium tuberculosis
ASJC Scopus subject areas
- Microbiology
- Immunology
- Microbiology (medical)
- Infectious Diseases
Cite this
Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51. / Al-Sayyed, Ban; Piperdi, Sajida; Yuan, Xinni; Li, Anping; Besra, Gurdyal S.; Jacobs, William R.; Casadevall, Arturo; Glatman-Freedman, Aharona.
In: Tuberculosis, Vol. 87, No. 6, 01.11.2007, p. 489-497.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51
AU - Al-Sayyed, Ban
AU - Piperdi, Sajida
AU - Yuan, Xinni
AU - Li, Anping
AU - Besra, Gurdyal S.
AU - Jacobs, William R.
AU - Casadevall, Arturo
AU - Glatman-Freedman, Aharona
PY - 2007/11/1
Y1 - 2007/11/1
N2 - Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
AB - Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.
KW - Monoclonal antibodies
KW - MPT51
KW - Mycobacterium tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=35348872454&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35348872454&partnerID=8YFLogxK
U2 - 10.1016/j.tube.2007.07.005
DO - 10.1016/j.tube.2007.07.005
M3 - Article
C2 - 17881291
AN - SCOPUS:35348872454
VL - 87
SP - 489
EP - 497
JO - Tuberculosis
JF - Tuberculosis
SN - 1472-9792
IS - 6
ER -