Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51

Ban Al-Sayyed; Sajida Piperdi; Xinni Yuan; Anping Li; Gurdyal S. Besra; William R. Jacobs; Arturo Casadevall; Aharona Glatman-Freedman

doi:10.1016/j.tube.2007.07.005

Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51

Ban Al-Sayyed, Sajida Piperdi, Xinni Yuan, Anping Li, Gurdyal S. Besra, William R. Jacobs, Arturo Casadevall, Aharona Glatman-Freedman

Microbiology & Immunology

Research output: Contribution to journal › Article

5 Scopus citations

Abstract

Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.

Original language	English (US)
Pages (from-to)	489-497
Number of pages	9
Journal	Tuberculosis
Volume	87
Issue number	6
DOIs	https://doi.org/10.1016/j.tube.2007.07.005
State	Published - Nov 1 2007

Keywords

MPT51
Monoclonal antibodies
Mycobacterium tuberculosis

ASJC Scopus subject areas

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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10.1016/j.tube.2007.07.005

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Al-Sayyed, B., Piperdi, S., Yuan, X., Li, A., Besra, G. S., Jacobs, W. R., Casadevall, A., & Glatman-Freedman, A. (2007). Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51. Tuberculosis, 87(6), 489-497. https://doi.org/10.1016/j.tube.2007.07.005

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abstract = "Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.",

keywords = "MPT51, Monoclonal antibodies, Mycobacterium tuberculosis",

author = "Ban Al-Sayyed and Sajida Piperdi and Xinni Yuan and Anping Li and Besra, {Gurdyal S.} and Jacobs, {William R.} and Arturo Casadevall and Aharona Glatman-Freedman",

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AU - Al-Sayyed, Ban

AU - Piperdi, Sajida

AU - Yuan, Xinni

AU - Li, Anping

AU - Besra, Gurdyal S.

AU - Jacobs, William R.

AU - Casadevall, Arturo

AU - Glatman-Freedman, Aharona

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AB - Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.

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KW - Monoclonal antibodies

KW - Mycobacterium tuberculosis

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