Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51

Ban Al-Sayyed, Sajida Piperdi, Xinni Yuan, Anping Li, Gurdyal S. Besra, William R. Jacobs, Arturo Casadevall, Aharona Glatman-Freedman

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Mycobacterium tuberculosis CDC 1551, a highly immunogenic outbreak strain, previously reported to have unique surface distribution of capsular polysaccharide, was used to generate novel monoclonal antibodies (mabs) to surface mycobacterial targets. Two immunoglobulin G1 (IgG1) mAbs, 16a1 and 16a6 were generated. The mAbs originated from the same B cell, bound strongly to whole cell M. tuberculosis CDC1551 and to its cell wall, membrane and cytosol fractions recognizing a 90 kDa protein. Immunoprecipitation using mAb 16a1 isolated a protein with amino acid peptide sequences matching MPT51 from the cytosol. This immunogenic protein of unknown function was previously reported only in culture filtrates of M. tuberculosis. Our findings suggest for the first time that this protein is found within the M. tuberculosis cell.

Original languageEnglish (US)
Pages (from-to)489-497
Number of pages9
JournalTuberculosis
Volume87
Issue number6
DOIs
StatePublished - Nov 2007

Keywords

  • MPT51
  • Monoclonal antibodies
  • Mycobacterium tuberculosis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

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    Al-Sayyed, B., Piperdi, S., Yuan, X., Li, A., Besra, G. S., Jacobs, W. R., Casadevall, A., & Glatman-Freedman, A. (2007). Monoclonal antibodies to Mycobacterium tuberculosis CDC 1551 reveal subcellular localization of MPT51. Tuberculosis, 87(6), 489-497. https://doi.org/10.1016/j.tube.2007.07.005