Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus

Livia C.L. Lopes, Rodrigo Rollin-Pinheiro, Allan J. Guimarães, Vera C.B. Bittencourt, Luis R. Martinez, Wade Koba, Sandra E. Farias, Joshua D. Nosanchuk, Eliana Barreto-Bergter

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Scedosporium apiospermum is part of the Pseudallescheria-Scedosporium complex. Peptidorhamnomannans (PRMs) are cell wall glycopeptides present in some fungi, and their structures have been characterized in S. apiospermum, S. prolificans and Sporothrix schenckii. Prior work shows that PRMs can interact with host cells and that the glycopeptides are antigenic. In the present study, three monoclonal antibodies (mAbs, IgG1) to S. apiospermum derived PRM were generated and their effects on S. apiospermum were examined in vitro and in vivo. The mAbs recognized a carbohydrate epitope on PRM. In culture, addition of the PRM mAbs increased S. apiospermum conidia germination and reduced conidial phagocytosis by J774.16 macrophages. In a murine infection model, mice treated with antibodies to PRM died prior to control animals. Thus, PRM is involved in morphogenesis and the binding of this glycopeptide by mAbs enhanced the virulence of the fungus. Further insights into the effects of these glycopeptides on the pathobiology of S. apiospermum may lead to new avenues for preventing and treating scedosporiosis.

Original languageEnglish (US)
Article numbere853
JournalPLoS neglected tropical diseases
Volume4
Issue number10
DOIs
StatePublished - Oct 2010

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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    Lopes, L. C. L., Rollin-Pinheiro, R., Guimarães, A. J., Bittencourt, V. C. B., Martinez, L. R., Koba, W., Farias, S. E., Nosanchuk, J. D., & Barreto-Bergter, E. (2010). Monoclonal antibodies against peptidorhamnomannans of Scedosporium apiospermum enhance the pathogenicity of the fungus. PLoS neglected tropical diseases, 4(10), [e853]. https://doi.org/10.1371/journal.pntd.0000853