Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia

L. L. Villa, L. Sichero, P. Rahal, O. Caballero, A. Ferenczy, Thomas E. Rohan, E. L. Franco

Research output: Contribution to journalArticle

229 Citations (Scopus)

Abstract

In order to determine geographically related intratypic variation in human papillomavirus (HPV) type 16 and 18 isolates that could be associated with lesion development, data were analysed from an ongoing cohort study of the natural course of infection of HPVs and cervical neoplasia. Testing for HPVs was carried out by PCR and molecular variants of these HPVs were characterized by sequence analysis of the long control region and by dot blot hybridization of the E6 and L1 genes. Tests for HPV were done in multiple first-year specimens from 1690 women enrolled in a cancer screening program from 1993 to 1997. Subjects were followed-up by cytology and cervicography for detection of cervical lesions. Seven variants of HPV-16 and four of HPV-18 were detected in one or more specimens from 65 subjects. The same variant was found in specimens taken on different visits from each case of persistent infection. Overall, non-European variants tended to persist more frequently [odds ratio (OR) = 4.5; 95% confidence interval (Cl), 1.6-12.4] than European (E) variants (OR = 2.5; 95% Cl, 1.3-4.9), relative to the risk of persistence for non-oncogenic HPVs. In addition, non-E variants were more strongly associated with risk of both prevalent (age- and race-adjusted OR = 172.2; 95% Cl, 47.1-630.1) and incident [relative risk (RR) = 22.5; 95% Cl, 6.0-83.9] high-grade lesions than E variants (prevalent lesions OR = 46.3; 95% Cl, 15.5-138.0 and incident lesons RR = 6.1; 95% Cl, 1.3-27.4), relative to the risk for HPV-negative women. Although consistent, the latter differences were not statistically significant. If confirmed in other populations, measurement of intratypic variation of HPV-16 and -18 has the potential to serve as an ancillary tool in cervical cancer screening.

Original languageEnglish (US)
Pages (from-to)2959-2968
Number of pages10
JournalJournal of General Virology
Volume81
Issue number12
StatePublished - 2000
Externally publishedYes

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Human papillomavirus 18
Human papillomavirus 16
Odds Ratio
Neoplasms
Early Detection of Cancer
Infection
Uterine Cervical Neoplasms
Sequence Analysis
Cell Biology
Cohort Studies
Confidence Intervals
Polymerase Chain Reaction
Population
Genes

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

Villa, L. L., Sichero, L., Rahal, P., Caballero, O., Ferenczy, A., Rohan, T. E., & Franco, E. L. (2000). Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia. Journal of General Virology, 81(12), 2959-2968.

Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia. / Villa, L. L.; Sichero, L.; Rahal, P.; Caballero, O.; Ferenczy, A.; Rohan, Thomas E.; Franco, E. L.

In: Journal of General Virology, Vol. 81, No. 12, 2000, p. 2959-2968.

Research output: Contribution to journalArticle

Villa, LL, Sichero, L, Rahal, P, Caballero, O, Ferenczy, A, Rohan, TE & Franco, EL 2000, 'Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia', Journal of General Virology, vol. 81, no. 12, pp. 2959-2968.
Villa, L. L. ; Sichero, L. ; Rahal, P. ; Caballero, O. ; Ferenczy, A. ; Rohan, Thomas E. ; Franco, E. L. / Molecular variants of human papillomavirus types 16 and 18 preferentially associated with cervical neoplasia. In: Journal of General Virology. 2000 ; Vol. 81, No. 12. pp. 2959-2968.
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