Molecular variants of human papillomavirus type 16 and 18 and risk for cervical neoplasia in Portugal

Angela Pista, Ana Oliveira, Andreia Barateiro, Helena Costa, Nuno Verdasca, Maria Teresa Paixão

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Persistent high-risk human papillomavirus (HPV) infection is considered as the central cause of invasive cervical cancer. Specific HPV 16 and 18 sequence variations were associated with an increased risk for progression. The purpose of this study was to analyze intratypic variations of HPV 16 and 18 within the E6 gene, MY09/11 and LCR regions, and to evaluate the risk of these variants for cervical neoplasia among Portuguese women. Cervical samples from 187 HPV 16-positive and 41 HPV 18-positive women with normal epithelium, cervical intraepithelial neoplasia, or invasive cervical cancer were amplified by type-specific PCR, followed by sequence and phylogenetic analysis. Sixteen new HPV 16 and 18 patterns are described in this paper. European HPV 16 variants were the most frequent (74.3%), particularly Ep-T350 (44.4%), followed by African (16.1%), and Asian-American (9.6%). Non-European HPV 16 variants were more frequent in pre-invasive lesions than in normal tissue and low-grade lesions. However, when analyzed separately, only African variants were associated significantly with an increased risk for cervical cancer. For HPV 18, the AsAi variant showed a trend, which was not statistically significant to an enhanced oncogenicity. European variants seemed to be significantly associated with a lower risk for cervical cancer development. The distribution of HPV 16 and 18 variants was not related to age or race among women living in the same geographical region. Knowledge of variants will be important for risk determination as well as for designing primers or probes for HPV detection methods, and for appropriate cervical cancer prevention strategies.

Original languageEnglish (US)
Pages (from-to)1889-1897
Number of pages9
JournalJournal of Medical Virology
Volume79
Issue number12
DOIs
StatePublished - Dec 2007
Externally publishedYes

Keywords

  • Cervical neoplasia
  • HPV 16
  • HPV 18
  • Intratypic variants
  • Portugal

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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