Molecular response of the patellar tendon to fatigue loading explained in the context of the initial induced damage and number of fatigue loading cycles

Nelly Andarawis-Puri, Jedd B. Sereysky, Hui (Herb) Sun, Karl J. Jepsen, Evan L. Flatow

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Accumulation of sub-rupture fatigue damage has been implicated in the development of tendinopathy. We previously developed an in vivo model of damage accumulation using the rat patellar tendon. Our model allows us to control the input loading parameters to induce fatigue damage in the tendon. Despite this precise control, the resulting induced damage could vary among animals because of differences in size or strength among their patellar tendons. In this study, we used number of applied cycles and initial (day-0) parameters that are indicative of induced damage to assess the molecular response 7 days after fatigue loading. We hypothesized that day-0 hysteresis, elongation, and stiffness of the loading and unloading load-displacement curves would be predictive of the 7-day molecular response. Results showed correlations between the 7-day molecular response and both day-0 elongation and unloading stiffness. Additionally, loading resulted in upregulation of several extracellular matrix genes that suggest adaptation; however, several of these genes (Col-I, -XII, MMP 2, and TIMP 3) shut down after a high level of damage was induced. We showed that evaluating the 7-day molecular profile in light of day-0 elongation provides important insight that is lost from comparing number of fatigue loading cycles only. Our data showed that loading generally results in an adaptive response. However, the tendon's ability to effectively respond deteriorates as greater damage is induced.

Original languageEnglish (US)
Pages (from-to)1327-1334
Number of pages8
JournalJournal of Orthopaedic Research
Volume30
Issue number8
DOIs
StatePublished - Aug 2012
Externally publishedYes

Fingerprint

Patellar Ligament
Fatigue
Tendons
Tissue Inhibitor of Metalloproteinase-3
Tendinopathy
Matrix Metalloproteinases
Genes
Extracellular Matrix
Rupture
Up-Regulation

Keywords

  • damage accumulation
  • elongation
  • molecular profile
  • stiffness
  • tendinopathy

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Molecular response of the patellar tendon to fatigue loading explained in the context of the initial induced damage and number of fatigue loading cycles. / Andarawis-Puri, Nelly; Sereysky, Jedd B.; Sun, Hui (Herb); Jepsen, Karl J.; Flatow, Evan L.

In: Journal of Orthopaedic Research, Vol. 30, No. 8, 08.2012, p. 1327-1334.

Research output: Contribution to journalArticle

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