Molecular Profile of Vascular Ion Channels after Experimental Subarachnoid Hemorrhage

Yasuo Aihara, Babak S. Jahromi, Reza Yassari, Elena Nikitina, Mayowa Agbaje-Williams, R. Loch Macdonald

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cerebral vasospasm is a transient, delayed constriction of cerebral arteries that occurs after subarachnoid hemorrhage (SAH). Smooth muscle cells show impaired relaxation after SAH, which may be caused by a defect in the ionic mechanisms regulating smooth muscle membrane potential and Ca 2+ permeability. We tested this hypothesis by examining changes in expression of mRNA and protein for ion channels in the basilar arteries of dogs after SAH using quantitative real-time polymerase chain reaction (PCR) and western blotting. SAH was associated with a significant reduction in basilar artery diameter to 41 ± 8% of pre-SAH diameter (P < 0.001) after 7 days. There was significant downregulation of the voltage-gated K+ channel Kv 2.2 (65% reduction in mRNA, P < 0.001; 49% reduction in protein, P < 0.05) and the β1 subunit of the large-conductance, Ca2+-activated K+ (BK) channel (53% reduction in mRNA, P < 0.02). There was no change in BK β1 subunit protein. Changes in mRNA levels of Kv 2.2 and the BK-β1 subunit correlated with the degree of vasospasm (r2 = 0.490 and 0.529 respectively, P < 0.05). The inwardly rectifying K+ (Kir) channel Kir 2.1 was upregulated (234% increase in mRNA, P < 0.001; 350% increase in protein, P < 0.001). There was no significant change in mRNA expression of L- type Ca2+ channels and the BK-α subunit. These data suggest that K+ channel dysfunction may contribute to the pathogenesis of cerebral vasospasm.

Original languageEnglish (US)
Pages (from-to)75-83
Number of pages9
JournalJournal of Cerebral Blood Flow and Metabolism
Volume24
Issue number1
StatePublished - Jan 2004
Externally publishedYes

Fingerprint

Subarachnoid Hemorrhage
Ion Channels
Blood Vessels
Messenger RNA
Large-Conductance Calcium-Activated Potassium Channels
Intracranial Vasospasm
Basilar Artery
Inwardly Rectifying Potassium Channel
Voltage-Gated Potassium Channels
Calcium-Activated Potassium Channels
Proteins
Cerebral Arteries
Protein Subunits
Constriction
Membrane Potentials
Smooth Muscle Myocytes
Smooth Muscle
Real-Time Polymerase Chain Reaction
Permeability
Down-Regulation

Keywords

  • Cerebral vasospasm
  • Gene expression
  • Potassium channels
  • Quantitative real-time RT-PCR
  • Subarachnoid hemorrhage

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Aihara, Y., Jahromi, B. S., Yassari, R., Nikitina, E., Agbaje-Williams, M., & Macdonald, R. L. (2004). Molecular Profile of Vascular Ion Channels after Experimental Subarachnoid Hemorrhage. Journal of Cerebral Blood Flow and Metabolism, 24(1), 75-83.

Molecular Profile of Vascular Ion Channels after Experimental Subarachnoid Hemorrhage. / Aihara, Yasuo; Jahromi, Babak S.; Yassari, Reza; Nikitina, Elena; Agbaje-Williams, Mayowa; Macdonald, R. Loch.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 24, No. 1, 01.2004, p. 75-83.

Research output: Contribution to journalArticle

Aihara, Y, Jahromi, BS, Yassari, R, Nikitina, E, Agbaje-Williams, M & Macdonald, RL 2004, 'Molecular Profile of Vascular Ion Channels after Experimental Subarachnoid Hemorrhage', Journal of Cerebral Blood Flow and Metabolism, vol. 24, no. 1, pp. 75-83.
Aihara, Yasuo ; Jahromi, Babak S. ; Yassari, Reza ; Nikitina, Elena ; Agbaje-Williams, Mayowa ; Macdonald, R. Loch. / Molecular Profile of Vascular Ion Channels after Experimental Subarachnoid Hemorrhage. In: Journal of Cerebral Blood Flow and Metabolism. 2004 ; Vol. 24, No. 1. pp. 75-83.
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