Molecular interaction and enzymatic activity of macrophage migration inhibitory factor with immunorelevant peptides

Ilaria Potolicchio, Laura Santambrogio, Jack L. Strominger

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Disulfide reduction is an important step in antigen processing for HLA class II restricted T cell responses. Migration inhibitory factor (MIF) is a member of the thioredoxin family and has been classically defined as a cytokine. Using enzyme-linked immunosorbent assay and CD analysis, here we describe the binding to MIF of two peptides, hepatitis B surface antigen (HBsAg) and insulin B (InsB) with high affinity for HLA class II allotypes, HLA-DP2 and HLA-DQ8, respectively. At neutral pH, cysteinylated InsB was a substrate for MIF thiol reductase activity, as assessed by mass spectroscopy/electrospray analysis. Finally, a biologically active form of MIF co-immunopurified with mature forms of HLA DP2/15, and a peptide derived from the HLA-DP β1 helix could be used for affinity purification of MIF. The possibility that MIF participates in class II antigen presentation and/or as a chaperone is discussed.

Original languageEnglish (US)
Pages (from-to)30889-30895
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number33
DOIs
StatePublished - Aug 15 2003
Externally publishedYes

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Macrophage Migration-Inhibitory Factors
Molecular interactions
Antigen Presentation
HLA-DP Antigens
Immunosorbents
Peptides
Thioredoxins
T-cells
Histocompatibility Antigens Class II
Hepatitis B Surface Antigens
Sulfhydryl Compounds
Disulfides
Purification
Assays
Mass Spectrometry
Oxidoreductases
Enzyme-Linked Immunosorbent Assay
Spectroscopy
Cytokines
T-Lymphocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular interaction and enzymatic activity of macrophage migration inhibitory factor with immunorelevant peptides. / Potolicchio, Ilaria; Santambrogio, Laura; Strominger, Jack L.

In: Journal of Biological Chemistry, Vol. 278, No. 33, 15.08.2003, p. 30889-30895.

Research output: Contribution to journalArticle

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