Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units

Akitoyo Hishimoto, Hiroko Nomaru, Qian K. Ye, Akira Nishi, Jihyeon Lim, Jennifer T. Aguilan, Edward Nieves, Gina Kang, Ruth Hogue Angeletti, Noboru Hiroi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) (MALDI-IMS) provides a technical means for simultaneous analysis of precise anatomic localization and regulation of peptides. We explored the technical capability of matrix-assisted laser desorption ionization mass spectrometry for characterization of peptidomic regulation by an addictive substance along two distinct projection systems in the mouse striatum. The spatial expression patterns of substance P and proenkephalin, marker neuropeptides of two distinct striatal projection neurons, were negatively correlated at baseline. We detected 768 mass/charge (m/z) peaks whose expression levels were mostly negatively and positively correlated with expression levels of substance P and proenkephalin A (amino acids 218-228), respectively, within the dorsal striatum. After nicotine administration, there was a positive shift in correlation of mass/charge peak expression levels with substance P and proenkephalin A (218-228). Our exploratory analyses demonstrate the technical capacity of MALDI-IMS for comprehensive identification of peptidomic regulation patterns along histochemically distinguishable striatal projection pathways.

Original languageEnglish (US)
JournalBiological Psychiatry
DOIs
StateAccepted/In press - Jul 15 2015

Fingerprint

Corpus Striatum
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Substance P
Neuropeptides
Nicotine
Neurons
Amino Acids
Peptides
proenkephalin

Keywords

  • Caudate-Putamen
  • In Situ Mass Spectrometry
  • Nicotine Addiction
  • Peptides
  • Peptidomics
  • Striatum

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units. / Hishimoto, Akitoyo; Nomaru, Hiroko; Ye, Qian K.; Nishi, Akira; Lim, Jihyeon; Aguilan, Jennifer T.; Nieves, Edward; Kang, Gina; Angeletti, Ruth Hogue; Hiroi, Noboru.

In: Biological Psychiatry, 15.07.2015.

Research output: Contribution to journalArticle

Hishimoto, Akitoyo ; Nomaru, Hiroko ; Ye, Qian K. ; Nishi, Akira ; Lim, Jihyeon ; Aguilan, Jennifer T. ; Nieves, Edward ; Kang, Gina ; Angeletti, Ruth Hogue ; Hiroi, Noboru. / Molecular Histochemistry Identifies Peptidomic Organization and Reorganization Along Striatal Projection Units. In: Biological Psychiatry. 2015.
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