TY - JOUR
T1 - Molecular detection of primary bladder cancer by microsatellite analysis
AU - Mao, Li
AU - Schoenberg, Mark P.
AU - Scicchitano, Marshall
AU - Erozan, Yener S.
AU - Merlo, Adrian
AU - Schwab, Donna
AU - Sidransky, David
PY - 1996/2/2
Y1 - 1996/2/2
N2 - Microsatellite DNA markers have been widely used as a tool for the detection of loss of heterozygosity and genomic instability in primary tumors. In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed by this molecular method and by conventional cytology, Microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients (95 percent) who were diagnosed with bladder cancer, whereas urine cytology detected cancer cells in 9 of 18 (50 percent) of the samples. These results suggest that microsatellite analysis, which in principle can be performed at about one-third the cost of cytology, may be a useful addition to current screening methods for detecting bladder cancer.
AB - Microsatellite DNA markers have been widely used as a tool for the detection of loss of heterozygosity and genomic instability in primary tumors. In a blinded study, urine samples from 25 patients with suspicious bladder lesions that had been identified cystoscopically were analyzed by this molecular method and by conventional cytology, Microsatellite changes matching those in the tumor were detected in the urine sediment of 19 of the 20 patients (95 percent) who were diagnosed with bladder cancer, whereas urine cytology detected cancer cells in 9 of 18 (50 percent) of the samples. These results suggest that microsatellite analysis, which in principle can be performed at about one-third the cost of cytology, may be a useful addition to current screening methods for detecting bladder cancer.
UR - http://www.scopus.com/inward/record.url?scp=0030034080&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030034080&partnerID=8YFLogxK
U2 - 10.1126/science.271.5249.659
DO - 10.1126/science.271.5249.659
M3 - Article
C2 - 8571131
AN - SCOPUS:0030034080
SN - 0036-8075
VL - 271
SP - 659
EP - 662
JO - Science
JF - Science
IS - 5249
ER -