Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21

Charles C. Clark, Isabelle Cohen, Inge Eichstetter, Linda A. Cannizzaro, John D. McPherson, John J. Wasmuth, Renato V. Iozzo

Research output: Contribution to journalArticle

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Abstract

The highly conserved Wnt genes belong to a widely distributed family of presumptive signaling molecules that have been implicated not only in the regulation of normal pattern formation during embryogenesis and differentiation of cell lineages, but also in oncogenic events. All of the known vertebrate Wnt genes encode for 38-to 43-kDa cysteine-rich putative glycoproteins, which have features typical of secreted growth factors: a hydrophobic signal sequence, a conserved asparagine-linked oligosaccharide consensus sequence, and 22 conserved cysteine residues whose relative spacing is maintained. In this study, we report the cloning and sequencing of several overlapping cDNAs encoding ∼4.1 kb of the human homologue of Wnt-5A. The mature protein contained 343 residues (Mr ∼ 38,000 excluding any post-translational modifications) with a >93% homology to the reported sequences of other Wnt-5A proteins (>99% homologous to mouse Wnt -5A). This protein maintained certain features-a hydrophobic signal sequence, the Wnt-1 family "signature sequence" (CKCHGvSGSC), and a number of other conserved amino acid residues: 24 cysteine residues, 4 asparagine-linked oligosaccharide consensus sequences, and a tyrosine sulfation site-that have been found in all other Wnt-5A proteins. Reverse transcriptase PCR analysis of RNA from a variety of human embryonic, neonatal, and adult cells and/or tissues showed that human Wnt-5A expression was detected only in neonatal heart and lung. It may be relevant, however, that the 3′-untranslated region contained numerous AT-rich motifs that could be involved in the rapid degradation of mRNA. Finally, using a combination of Southern blotting, PCR amplification, and in situ hybridization, the human Wnt-5A (WNT5A) gene was mapped to chromosome 3p14-p21.

Original languageEnglish (US)
Pages (from-to)249-260
Number of pages12
JournalGenomics
Volume18
Issue number2
DOIs
StatePublished - Nov 1993
Externally publishedYes

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Proto-Oncogenes
Chromosome Mapping
Molecular Cloning
Chromosomes
Cysteine
Asparagine
Consensus Sequence
Protein Sorting Signals
Oligosaccharides
Genes
RNA Stability
3' Untranslated Regions
Cell Lineage
Post Translational Protein Processing
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Embryonic Development
In Situ Hybridization
Tyrosine
Vertebrates

ASJC Scopus subject areas

  • Genetics

Cite this

Clark, C. C., Cohen, I., Eichstetter, I., Cannizzaro, L. A., McPherson, J. D., Wasmuth, J. J., & Iozzo, R. V. (1993). Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21. Genomics, 18(2), 249-260. https://doi.org/10.1006/geno.1993.1463

Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21. / Clark, Charles C.; Cohen, Isabelle; Eichstetter, Inge; Cannizzaro, Linda A.; McPherson, John D.; Wasmuth, John J.; Iozzo, Renato V.

In: Genomics, Vol. 18, No. 2, 11.1993, p. 249-260.

Research output: Contribution to journalArticle

Clark, CC, Cohen, I, Eichstetter, I, Cannizzaro, LA, McPherson, JD, Wasmuth, JJ & Iozzo, RV 1993, 'Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21', Genomics, vol. 18, no. 2, pp. 249-260. https://doi.org/10.1006/geno.1993.1463
Clark CC, Cohen I, Eichstetter I, Cannizzaro LA, McPherson JD, Wasmuth JJ et al. Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21. Genomics. 1993 Nov;18(2):249-260. https://doi.org/10.1006/geno.1993.1463
Clark, Charles C. ; Cohen, Isabelle ; Eichstetter, Inge ; Cannizzaro, Linda A. ; McPherson, John D. ; Wasmuth, John J. ; Iozzo, Renato V. / Molecular Cloning of the Human Proto-oncogene Wnt-5A and Mapping of the Gene (WNT5A) to Chromosome 3p14-p21. In: Genomics. 1993 ; Vol. 18, No. 2. pp. 249-260.
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abstract = "The highly conserved Wnt genes belong to a widely distributed family of presumptive signaling molecules that have been implicated not only in the regulation of normal pattern formation during embryogenesis and differentiation of cell lineages, but also in oncogenic events. All of the known vertebrate Wnt genes encode for 38-to 43-kDa cysteine-rich putative glycoproteins, which have features typical of secreted growth factors: a hydrophobic signal sequence, a conserved asparagine-linked oligosaccharide consensus sequence, and 22 conserved cysteine residues whose relative spacing is maintained. In this study, we report the cloning and sequencing of several overlapping cDNAs encoding ∼4.1 kb of the human homologue of Wnt-5A. The mature protein contained 343 residues (Mr ∼ 38,000 excluding any post-translational modifications) with a >93{\%} homology to the reported sequences of other Wnt-5A proteins (>99{\%} homologous to mouse Wnt -5A). This protein maintained certain features-a hydrophobic signal sequence, the Wnt-1 family {"}signature sequence{"} (CKCHGvSGSC), and a number of other conserved amino acid residues: 24 cysteine residues, 4 asparagine-linked oligosaccharide consensus sequences, and a tyrosine sulfation site-that have been found in all other Wnt-5A proteins. Reverse transcriptase PCR analysis of RNA from a variety of human embryonic, neonatal, and adult cells and/or tissues showed that human Wnt-5A expression was detected only in neonatal heart and lung. It may be relevant, however, that the 3′-untranslated region contained numerous AT-rich motifs that could be involved in the rapid degradation of mRNA. Finally, using a combination of Southern blotting, PCR amplification, and in situ hybridization, the human Wnt-5A (WNT5A) gene was mapped to chromosome 3p14-p21.",
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