Molecular cloning of murine folylpoly-γ-glutamate synthetase

Michael J. Spinella, Kevin E. Brigle, I. David Goldman

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Folylpoly-γ-glutamate synthetase (FPGS) is essential for mammalian cell survival and is a major determinant of cytotoxicity and selectivity for folate antimetabolites. Here we describe the cloning of a cDNA encoding murine FPGS isolated from L1210 leukemia cells. The amino acid sequence of murine FPGS is 82% identical to human FPGS [1] with identical discrete regions of up to 41 residues. Murine FPGS contains two AUG initiation codons, shown to be responsible for mitochondrial and cytosolic forms of the enzyme in human cells [2]. Previous studies indicated species, tissue, and tumor specific differences in mammalian FPGS. The availability of murine FPGS expands the knowledge and understanding of the spectrum of these variations.

Original languageEnglish (US)
Pages (from-to)11-14
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1305
Issue number1-2
DOIs
StatePublished - Feb 7 1996
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Ligases
Glutamic Acid
Cells
Leukemia L1210
Antimetabolites
Initiator Codon
Cytotoxicity
Folic Acid
Organism Cloning
Tumors
Amino Acid Sequence
Cell Survival
Complementary DNA
Availability
Tissue
Amino Acids
Enzymes
Neoplasms

Keywords

  • cDNA (murine)
  • Folylpolyglutamate synthetase
  • L1210

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular cloning of murine folylpoly-γ-glutamate synthetase. / Spinella, Michael J.; Brigle, Kevin E.; Goldman, I. David.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1305, No. 1-2, 07.02.1996, p. 11-14.

Research output: Contribution to journalArticle

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