Molecular cloning of LMO4, a new human LIM domain gene

Janis Racevskis; Alyssa Dill; Joseph A. Sparano; Handan Ruan

doi:10.1016/S0167-4781(99)00037-8

Molecular cloning of LMO4, a new human LIM domain gene

Janis Racevskis, Alyssa Dill, Joseph A. Sparano, Handan Ruan

Medicine

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

We have identified a new human LIM domain gene by isolating an autoantigenic cDNA clone from a human breast tumor cDNA library. The predicted amino acid sequence of the cDNA clone's 495 bp open reading frame contains two tandem LIM domain motifs, and within the LIM domain region there is 62% identity with the analogous region of the LIM-only gene LMO1. The homology to LMO1 is restricted to the 360 bp region encoding the tandemly repeated LIM domains, the rest of the open reading frame as well as the extensive, GC-rich 5' untranslated region, and 3' region of the 2 kb cDNA sequence are unrelated to any known genes. This gene has been designated LMO4.

Original language	English (US)
Pages (from-to)	148-153
Number of pages	6
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1445
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(99)00037-8
State	Published - Apr 14 1999

Keywords

CDNA sequence
Human
LIM domain
LMO4

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/S0167-4781(99)00037-8

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title = "Molecular cloning of LMO4, a new human LIM domain gene",

abstract = "We have identified a new human LIM domain gene by isolating an autoantigenic cDNA clone from a human breast tumor cDNA library. The predicted amino acid sequence of the cDNA clone's 495 bp open reading frame contains two tandem LIM domain motifs, and within the LIM domain region there is 62% identity with the analogous region of the LIM-only gene LMO1. The homology to LMO1 is restricted to the 360 bp region encoding the tandemly repeated LIM domains, the rest of the open reading frame as well as the extensive, GC-rich 5' untranslated region, and 3' region of the 2 kb cDNA sequence are unrelated to any known genes. This gene has been designated LMO4.",

keywords = "CDNA sequence, Human, LIM domain, LMO4",

author = "Janis Racevskis and Alyssa Dill and Sparano, {Joseph A.} and Handan Ruan",

note = "Funding Information: This work was funded by Grant DAMD17-94-J-4386 from the US Army Medical Research and Development Command, Breast Cancer Research Program (to JR). Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.",

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AU - Dill, Alyssa

AU - Sparano, Joseph A.

AU - Ruan, Handan

N1 - Funding Information: This work was funded by Grant DAMD17-94-J-4386 from the US Army Medical Research and Development Command, Breast Cancer Research Program (to JR). Copyright: Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

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N2 - We have identified a new human LIM domain gene by isolating an autoantigenic cDNA clone from a human breast tumor cDNA library. The predicted amino acid sequence of the cDNA clone's 495 bp open reading frame contains two tandem LIM domain motifs, and within the LIM domain region there is 62% identity with the analogous region of the LIM-only gene LMO1. The homology to LMO1 is restricted to the 360 bp region encoding the tandemly repeated LIM domains, the rest of the open reading frame as well as the extensive, GC-rich 5' untranslated region, and 3' region of the 2 kb cDNA sequence are unrelated to any known genes. This gene has been designated LMO4.

AB - We have identified a new human LIM domain gene by isolating an autoantigenic cDNA clone from a human breast tumor cDNA library. The predicted amino acid sequence of the cDNA clone's 495 bp open reading frame contains two tandem LIM domain motifs, and within the LIM domain region there is 62% identity with the analogous region of the LIM-only gene LMO1. The homology to LMO1 is restricted to the 360 bp region encoding the tandemly repeated LIM domains, the rest of the open reading frame as well as the extensive, GC-rich 5' untranslated region, and 3' region of the 2 kb cDNA sequence are unrelated to any known genes. This gene has been designated LMO4.

KW - CDNA sequence

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KW - LIM domain

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Molecular cloning of LMO4, a new human LIM domain gene

Abstract

Keywords

ASJC Scopus subject areas

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