Molecular cloning of human thiamin pyrophosphokinase

Rongbao Zhao; Feng Gao; I. David Goldman

doi:10.1016/S0167-4781(00)00264-5

Molecular cloning of human thiamin pyrophosphokinase

Rongbao Zhao, Feng Gao, I. David Goldman

Oncology

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state.

Original language	English (US)
Pages (from-to)	320-322
Number of pages	3
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1517
Issue number	2
DOIs	https://doi.org/10.1016/S0167-4781(00)00264-5
State	Published - Jan 26 2001

Keywords

Cofactor
Pyrophosphate
Pyrophosphokinase
Thiamin

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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title = "Molecular cloning of human thiamin pyrophosphokinase",

abstract = "Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state.",

keywords = "Cofactor, Pyrophosphate, Pyrophosphokinase, Thiamin",

author = "Rongbao Zhao and Feng Gao and Goldman, {I. David}",

note = "Funding Information: This work was supported by National Cancer Institute Grants CA-39807 and CA82621 (I.D.G.).",

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T1 - Molecular cloning of human thiamin pyrophosphokinase

AU - Zhao, Rongbao

AU - Gao, Feng

AU - Goldman, I. David

N1 - Funding Information: This work was supported by National Cancer Institute Grants CA-39807 and CA82621 (I.D.G.).

PY - 2001/1/26

Y1 - 2001/1/26

N2 - Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state.

AB - Thiamin pyrophosphokinase (TPK, EC 2.7.6.2) catalyses phosphorylation of thiamin to thiamin pyrophosphate, an active enzyme cofactor. Here we describe the cloning of complete human TPK1 cDNA from an adult liver library. Human TPK1 is 89% identical to murine TPK1 at the protein level. The gene maps to chromosome 7q34-36, consists of at least eight exons, and spans a distance at least of 420 kb. The mRNA of human TPK1 is highly expressed in testis, small intestine and kidney with lesser but detectable expression in brain, liver, placenta and spleen. The availability of the human TPK1 gene will provide another useful tool for studying the role of this enzyme in human thiamin metabolism and deficiency state.

KW - Cofactor

KW - Pyrophosphate

KW - Pyrophosphokinase

KW - Thiamin

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IS - 2

ER -

Molecular cloning of human thiamin pyrophosphokinase

Abstract

Keywords

ASJC Scopus subject areas

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