Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse

Jack Lenz, R. Crowther, S. Klimenko, W. Haseltine

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

SL3-3 is a leukemogenic, ecotropic retrovirus produced by a T-cell line derived from a spontaneous lymphoma of an AKR mouse. We have isolated a molecular clone of its DNA provirus from infected NIH 3T3 fibroblasts. Cloned proviral DNA produced infectious virus upon transfection onto NIH 3T3 cells. Virus derived by transfection induced lymphomas at high frequency in AKR/J, C3Hf/Bi, CBA/J, and NFS/N mice. Heteroduplex and RNase T1 fingerprinting analyses showed that the genomes of SL3-3 and the non-leukemogenic virus, Akv, contain no major substitutions relative to one another and differ by only a few base changes. These results unambiguously show that SL3-3 is a highly leukemogenic virus and that major rearrangements of the genome relative to Akv are not required for virulence.

Original languageEnglish (US)
Pages (from-to)943-951
Number of pages9
JournalJournal of Virology
Volume43
Issue number3
StatePublished - 1982
Externally publishedYes

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Retroviridae
Inbred AKR Mouse
Molecular Cloning
molecular cloning
Viruses
viruses
Transfection
Lymphoma
mice
transfection
lymphoma
Genome
Ribonuclease T1
Proviruses
NIH 3T3 Cells
DNA
proviruses
genome
Virulence
Clone Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Lenz, J., Crowther, R., Klimenko, S., & Haseltine, W. (1982). Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse. Journal of Virology, 43(3), 943-951.

Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse. / Lenz, Jack; Crowther, R.; Klimenko, S.; Haseltine, W.

In: Journal of Virology, Vol. 43, No. 3, 1982, p. 943-951.

Research output: Contribution to journalArticle

Lenz, J, Crowther, R, Klimenko, S & Haseltine, W 1982, 'Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse', Journal of Virology, vol. 43, no. 3, pp. 943-951.
Lenz, Jack ; Crowther, R. ; Klimenko, S. ; Haseltine, W. / Molecular cloning of a highly leukemogenic, ecotropic retrovirus from an AKR mouse. In: Journal of Virology. 1982 ; Vol. 43, No. 3. pp. 943-951.
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