Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2

Guanqing Wu, Toshio Mochizuki, Thanh C. Le, Yiqiang Cai, Tomohito Hayashi, David M. Reynolds, Stefan Somlo

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recertify been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned CDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH2 and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin- 2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.

Original languageEnglish (US)
Pages (from-to)220-223
Number of pages4
JournalGenomics
Volume45
Issue number1
DOIs
StatePublished - Oct 1 1997

ASJC Scopus subject areas

  • Genetics

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