Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2

Guanqing Wu; Toshio Mochizuki; Thanh C. Le; Yiqiang Cai; Tomohito Hayashi; David M. Reynolds; Stefan Somlo

doi:10.1006/geno.1997.4920

Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2

Guanqing Wu, Toshio Mochizuki, Thanh C. Le, Yiqiang Cai, Tomohito Hayashi, David M. Reynolds, Stefan Somlo

Genetics

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recertify been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned CDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH₂ and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin- 2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.

Original language	English (US)
Pages (from-to)	220-223
Number of pages	4
Journal	Genomics
Volume	45
Issue number	1
DOIs	https://doi.org/10.1006/geno.1997.4920
State	Published - Oct 1 1997

ASJC Scopus subject areas

Genetics

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title = "Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2",

abstract = "The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recertify been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned CDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH2 and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin- 2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.",

author = "Guanqing Wu and Toshio Mochizuki and Le, {Thanh C.} and Yiqiang Cai and Tomohito Hayashi and Reynolds, {David M.} and Stefan Somlo",

note = "Funding Information: We thank Mary Barter and Lucy Rowe of The Jackson Laboratory for the analysis of the backcross panel data and George Grills for help with sequencing. This research was supported by grants from the NIH (DK02015) and the March of Dimes Birth Defects Foundation. Sequencing support was provided by the Albert Einstein College of Medicine Human Genome Program.",

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doi = "10.1006/geno.1997.4920",

language = "English (US)",

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AU - Wu, Guanqing

AU - Mochizuki, Toshio

AU - Le, Thanh C.

AU - Cai, Yiqiang

AU - Hayashi, Tomohito

AU - Reynolds, David M.

AU - Somlo, Stefan

N1 - Funding Information: We thank Mary Barter and Lucy Rowe of The Jackson Laboratory for the analysis of the backcross panel data and George Grills for help with sequencing. This research was supported by grants from the NIH (DK02015) and the March of Dimes Birth Defects Foundation. Sequencing support was provided by the Albert Einstein College of Medicine Human Genome Program.

PY - 1997/10/1

Y1 - 1997/10/1

N2 - The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recertify been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned CDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH2 and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin- 2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.

AB - The gene responsible for the second form of autosomal dominant polycystic kidney disease, PKD2, has recertify been identified. We now describe the cloning, genomic localization, cDNA sequence, and expression analysis of its murine homologue, Pkd2. The cloned CDNA sequence is 5134 bp long and is predicted to encode a 966-amino-acid integral membrane protein with six membrane-spanning domains and intracellular NH2 and COOH termini. Pkd2 is highly conserved with 91% identity and 98% similarity to polycystin- 2 at the amino acid level. Pkd2 mRNA is widely expressed in mouse tissues. Pkd2 maps to mouse chromosome 5 and is excluded as a candidate gene for previously mapped mouse mutations resulting in a polycystic kidney phenotype.

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Molecular cloning, cDNA sequence analysis, and chromosomal localization of mouse Pkd2

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