Molecular cloning and functional expression of the cytochrome P450 11B-hydroxylase of the guinea pig

Hannes E. Buelow, Katharina Möbius, Volker Bähr, Rita Bernhardt

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A cDNA library has been constructed from adrenal glands of guinea pigs receiving a low sodium/high potassium diet. From this library a complementary DNA encoding an 11β-hydroxylase (EC 1.14.15.4) was isolated. Northern blot analysis revealed a major transcript of 2.2 kb size. The cDNA was cloned into an eucaryotic expression vector and the activity of the enzyme towards various substrates was investigated after expression in COS-1 cells. The enzyme was able to perform 11β-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstendione as well as 18-hydroxylations and 19-hydroxylations to a small extent. Phylogenetic analyses with thirteen protein sequences of the CYP11B subfamily showed an early divergence of the guinea pig, but could not establish a definite branching order.

Original languageEnglish (US)
Pages (from-to)304-312
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number2
DOIs
StatePublished - Apr 16 1996
Externally publishedYes

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Hydroxylation
Cloning
Molecular Cloning
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Guinea Pigs
Complementary DNA
Cortodoxone
Desoxycorticosterone
COS Cells
Enzymes
Nutrition
Adrenal Glands
Gene Library
Northern Blotting
Libraries
Potassium
Sodium
Diet
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning and functional expression of the cytochrome P450 11B-hydroxylase of the guinea pig. / Buelow, Hannes E.; Möbius, Katharina; Bähr, Volker; Bernhardt, Rita.

In: Biochemical and Biophysical Research Communications, Vol. 221, No. 2, 16.04.1996, p. 304-312.

Research output: Contribution to journalArticle

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