Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6

Kausik Si, Jayanta Chaudhuri, Jorge Chevesich, Umadas Maitra

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Eukaryotic translation initiation factor 6 (eIF6) binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. In this paper, we devised a procedure for purifying eIF6 from rabbit reticulocyte lysates and immunochemically characterized the protein by using antibodies isolated from egg yolks of laying hens immunized with rabbit eIF6. By using these monospecific antibodies, a 1.096-kb human cDNA that encodes an eIF6 of 245 amino acids (calculated M(r) 26,558) has been cloned and expressed in Escherichia coli. The purified recombinant human protein exhibits biochemical properties that are similar to eIF6 isolated from mammalian cell extracts. Database searches identified amino acid sequences from Saccharomyces cerevisiae, Drosophila, and the nematode Caenorhabditis elegans with significant identity to the deduced amino acid sequence of human eIF6, suggesting the presence of homologues of human eIF6 in these organisms.

Original languageEnglish (US)
Pages (from-to)14285-14290
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number26
DOIs
StatePublished - Dec 23 1997

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Eukaryotic Initiation Factors
Peptide Initiation Factors
Molecular Cloning
Complementary DNA
Amino Acid Sequence
Eukaryotic Large Ribosome Subunits
Eukaryotic Small Ribosome Subunits
Rabbits
Egg Yolk
Antibodies
Reticulocytes
Caenorhabditis elegans
eIF-6
Cell Extracts
Recombinant Proteins
Drosophila
Saccharomyces cerevisiae
Databases
Escherichia coli
Amino Acids

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Molecular cloning and functional expression of a human cDNA encoding translation initiation factor 6. / Si, Kausik; Chaudhuri, Jayanta; Chevesich, Jorge; Maitra, Umadas.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 26, 23.12.1997, p. 14285-14290.

Research output: Contribution to journalArticle

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