Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

Kallol Das, Jorge Chevesich, Umadas Maitra

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

Original languageEnglish (US)
Pages (from-to)3058-3062
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number7
DOIs
Publication statusPublished - Apr 1 1993

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Keywords

  • GTP hydrolysis
  • GTPase superfamily proteins
  • Protein synthesis

ASJC Scopus subject areas

  • General

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