Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

Kallol Das, Jorge Chevesich, Umadas Maitra

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

Original languageEnglish (US)
Pages (from-to)3058-3062
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number7
StatePublished - Apr 1 1993

Fingerprint

Eukaryotic Initiation Factor-5
Eukaryotic Initiation Factors
Peptide Initiation Factors
Molecular Cloning
Complementary DNA
Eukaryotic Large Ribosome Subunits
Amino Acid Motifs
GTP Phosphohydrolases
Reticulocytes
Guanosine Triphosphate
Amino Acid Sequence
Proteins
Hydrolysis
Escherichia coli
Rabbits
Amino Acids

Keywords

  • GTP hydrolysis
  • GTPase superfamily proteins
  • Protein synthesis

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Molecular cloning and expression of cDNA for mammalian translation initiation factor 5. / Das, Kallol; Chevesich, Jorge; Maitra, Umadas.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 7, 01.04.1993, p. 3058-3062.

Research output: Contribution to journalArticle

@article{727073e290854263839b4f3f519b0c3d,
title = "Molecular cloning and expression of cDNA for mammalian translation initiation factor 5",
abstract = "Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.",
keywords = "GTP hydrolysis, GTPase superfamily proteins, Protein synthesis",
author = "Kallol Das and Jorge Chevesich and Umadas Maitra",
year = "1993",
month = "4",
day = "1",
language = "English (US)",
volume = "90",
pages = "3058--3062",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - Molecular cloning and expression of cDNA for mammalian translation initiation factor 5

AU - Das, Kallol

AU - Chevesich, Jorge

AU - Maitra, Umadas

PY - 1993/4/1

Y1 - 1993/4/1

N2 - Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

AB - Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S·AUG·Met-tRNAf-eIF-2·GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated Mr 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

KW - GTP hydrolysis

KW - GTPase superfamily proteins

KW - Protein synthesis

UR - http://www.scopus.com/inward/record.url?scp=0027497970&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027497970&partnerID=8YFLogxK

M3 - Article

VL - 90

SP - 3058

EP - 3062

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -