Molecular cloning and characterization of mitogen-activated protein kinase 2 in Trypanosoma cruzi

Yi Bao, Louis M. Weiss, Yan Fen Ma, Michael P. Lisanti, Herbert B. Tanowitz, Bhaskar C. Das, Renjian Zheng, Huan Huang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Mitogen-activated protein kinase (MApK) pathways are major signal transduction systems by which eukaryotic cells convert environmental cues to intracellular events such as proliferation and differentiation. We have identified a Trypanosoma cruzi homologue of the MApK family that we have called tcMApK2. Sequence analyses demonstrates tcMApK2 has high homology with lower eukaryotic eRK2 but has significant differences from mammalian eRK2. enzymatic assays of both recombinant tcMApK2 and native protein obtained by immunoprecipitation using anti-tcMApK2 demonstrated that both preparations of tcMApK2 were catalytically active. Immunofluorescence analysis of the subcellular localization of tcMApK2 determined it is mainly cytoplasmic in epimastigotes, along the flagella in trypomastigotes and on the plasma membrane of intracellular amastigotes. phosphorylated tcMApK2 was highest in trypomastigotes and lowest in amastigotes. Recombinant tcMApK2 was able to phosphorylate the recombinant protein of a cAMp specific phosphodiesterase. overexpression of tcMApK2 in epimastigotes inhibited growth and development leading to death. tcMApK2 has an important role in the stress response of the parasite and may be important in regulating proliferation and differentiation.

Original languageEnglish (US)
Pages (from-to)2888-2896
Number of pages9
JournalCell Cycle
Volume9
Issue number14
DOIs
StatePublished - Jul 15 2010
Externally publishedYes

Fingerprint

Trypanosoma cruzi
Mitogen-Activated Protein Kinase 1
Molecular Cloning
Mitogen-Activated Protein Kinases
Flagella
Phosphoric Diester Hydrolases
Enzyme Assays
Eukaryotic Cells
Growth and Development
Immunoprecipitation
Recombinant Proteins
Cues
Fluorescent Antibody Technique
Sequence Analysis
Signal Transduction
Parasites
Cell Membrane
Proteins

Keywords

  • Mitogen-activated protein kinase
  • Phosphorylation
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Developmental Biology

Cite this

Molecular cloning and characterization of mitogen-activated protein kinase 2 in Trypanosoma cruzi. / Bao, Yi; Weiss, Louis M.; Ma, Yan Fen; Lisanti, Michael P.; Tanowitz, Herbert B.; Das, Bhaskar C.; Zheng, Renjian; Huang, Huan.

In: Cell Cycle, Vol. 9, No. 14, 15.07.2010, p. 2888-2896.

Research output: Contribution to journalArticle

Bao, Yi ; Weiss, Louis M. ; Ma, Yan Fen ; Lisanti, Michael P. ; Tanowitz, Herbert B. ; Das, Bhaskar C. ; Zheng, Renjian ; Huang, Huan. / Molecular cloning and characterization of mitogen-activated protein kinase 2 in Trypanosoma cruzi. In: Cell Cycle. 2010 ; Vol. 9, No. 14. pp. 2888-2896.
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