Molecular cloning and characterization of a cDNA for human granulocyte colony-stimulating factor (G-CSF) from a glioblastoma multiforme cell line and localization of the G-CSF gene to chromosome band 17q21

D. J. Tweardy, L. A. Cannizzaro, A. P. Palumbo, S. Shane, K. Huebner, P. Vantuinen, D. H. Ledbetter, J. B. Finan, P. C. Nowell, G. Rovera

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32 Scopus citations

Abstract

The conditioned media (CM) of the glioblastoma multiforme cell line, U87 MG, contains abundant granulocyte colony-stimulating factor (G-CSF) activity (Tweardy et al., 1987). An oligonucleotide encoding the amino acids -11 to -4 of G-CSF detected a single abundant G-CSF mRNA of 1.6 kilobases (Kb) produced by U87 MG cells. Screening of a U87 MG cDNA library with the oligonucleotide identified cDNA clones of 1.3-1.4 Kb. Sequencing of one clone (pG-CSF6) confirmed that it encoded G-CSF and was derived from G-CSFb mRNA encoding a protein with a three amino acid deletion at positions 36-38. Only a single base substitution was observed at the third position of the codon for leu 152 indicating that G-CSF is highly conserved in cells of widely different origin. Somatic cell hybridization studies and chromosomal in situ hybridization localized the G-CSF gene to the long arm of chromosome 17 in band 17q21, proximal to the 17q breakpoint characteristic of acute promyelocytic leukemia.

Original languageEnglish (US)
Pages (from-to)209-220
Number of pages12
JournalOncogene Research
Volume1
Issue number3
StatePublished - Dec 1 1987

ASJC Scopus subject areas

  • Cancer Research

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    Tweardy, D. J., Cannizzaro, L. A., Palumbo, A. P., Shane, S., Huebner, K., Vantuinen, P., Ledbetter, D. H., Finan, J. B., Nowell, P. C., & Rovera, G. (1987). Molecular cloning and characterization of a cDNA for human granulocyte colony-stimulating factor (G-CSF) from a glioblastoma multiforme cell line and localization of the G-CSF gene to chromosome band 17q21. Oncogene Research, 1(3), 209-220.