Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent

R. Berland, E. Fikrig, D. Rahn, J. Hardin, R. A. Flavell

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The earliest humoral response in patients infected with Borrelia burgdorferi, the agent of Lyme disease, is directed against the spirochete's 41-kDa flagellar antigen. In order to map the epitopes recognized on this antigen, 11 overlapping fragments spanning the flagellin gene were cloned by polymerase chain reaction and inserted into an Escherichia coli expression vector which directed their expression as fusion proteins containing glutathione S-transferase at the N terminus and a flagellin fragment at the C terminus. Affinity-purified fusion proteins were assayed for reactivity on Western blots (immunoblots) with sera from patients with late-stage Lyme disease. The same immunodominant domain was bound by sera from 17 of 18 patients. This domain (comprising amino acids 197 to 241) does not share significant homology with other bacterial flagellins and therefore may be useful in serological testing for Lyme disease.

Original languageEnglish (US)
Pages (from-to)3531-3535
Number of pages5
JournalInfection and Immunity
Volume59
Issue number10
StatePublished - 1991
Externally publishedYes

Fingerprint

Flagellin
Borrelia burgdorferi
Lyme Disease
Antigens
Western Blotting
Immunodominant Epitopes
Spirochaetales
Glutathione Transferase
Serum
Epitopes
Proteins
Escherichia coli
Amino Acids
Polymerase Chain Reaction
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent. / Berland, R.; Fikrig, E.; Rahn, D.; Hardin, J.; Flavell, R. A.

In: Infection and Immunity, Vol. 59, No. 10, 1991, p. 3531-3535.

Research output: Contribution to journalArticle

Berland, R. ; Fikrig, E. ; Rahn, D. ; Hardin, J. ; Flavell, R. A. / Molecular characterization of the humoral response to the 41-kilodalton flagellar antigen of Borrelia burgdorferi, the Lyme disease agent. In: Infection and Immunity. 1991 ; Vol. 59, No. 10. pp. 3531-3535.
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