Abstract
The hybridoma technology allows the production of large quantities of specific antibodies of a single isotype. Since different isotypes have special effector functions and are distributed distinctively throughout the body, it is often useful to have a library of switch variants from the original monoclonal antibody. We have shown previously that forced expression of activation induced cytidine deaminase (AID) in hybridomas increased their very low frequency of class switch recombination (CSR) in vitro only ∼ 7-13 fold. Since we had previously identified rare hybridoma subclones that spontaneously switched at more than 100 times higher frequencies, we have now examined those higher switching variants to search for ways to further increase the frequency of isotype switching in vitro. AID was not responsible for the ∼ 100 fold increase in CSR, so we used whole-genome gene expression profiling to provide a platform for studying candidate molecular pathways underlying spontaneous CSR in hybridomas.
Original language | English (US) |
---|---|
Pages (from-to) | 71-78 |
Number of pages | 8 |
Journal | Journal of Immunological Methods |
Volume | 350 |
Issue number | 1-2 |
DOIs | |
State | Published - Oct 31 2009 |
Keywords
- AID
- Class switch recombination
- Hybridoma
- Immunoglobulin
- Microarrays
- Real-time RT-PCR
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology