Molecular characterization of a novel, developmentally regulated small embryonic chaperone from Caenorhabditis elegans

Barbara Linder, Zhijun Jin, Jonathan H. Freedman, Charles S. Rubin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Low molecular weight chaperones inhibit protein aggregation and facilitate refolding of partially denatured polypeptides in cells subjected to physical and chemical stresses. The nematode Caenorhabditis elegans provides a system amenable for investigations on roles for chaperone proteins in normal homeostasis and development. We characterized a C. elegans gene and cDNAs that encode a novel, small embryonic chaperone-like protein (SEC-1) that is composed of 159 amino acids. The central core of SEC-1 (residues 45- 126) is ~40% identical with a corresponding segment of mammalian Hsp27 and αB crystallin. Expression of SEC-1 in Escherichia coli confers thermotolerance on the bacterium. SEC-1 mRNA is evident only in C. elegans oocytes and developing embryos. Translation and accumulation of SEC-1 protein is temporally coupled with a prolonged burst of intense protein synthesis and rapid mitogenesis during early embryogenesis. As the rate of protein synthesis decreases during late embryogenesis, levels of SEC-1 and its cognate mRNA decline precipitously. Induction/deinduction of SEC-1 is precisely regulated by intrinsic developmental factors rather than extrinsic stresses. In vivo injection of C. elegans oocytes with antisense oligonucleotides that complement the 5'-end of SEC-1 mRNA arrests nematode development at an early stage after fertilization. Thus, SEC-1 appears to be adapted to perform essential functions in early embryogenesis.

Original languageEnglish (US)
Pages (from-to)30158-30166
Number of pages9
JournalJournal of Biological Chemistry
Volume271
Issue number47
DOIs
StatePublished - 1996

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Caenorhabditis elegans
Embryonic Development
Proteins
Messenger RNA
Oocytes
Complement C5
Intrinsic Factor
Crystallins
Molecular Chaperones
Antisense Oligonucleotides
Fertilization
Escherichia coli
Bacteria
Homeostasis
Agglomeration
Embryonic Structures
Complementary DNA
Genes
Molecular Weight
Molecular weight

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular characterization of a novel, developmentally regulated small embryonic chaperone from Caenorhabditis elegans. / Linder, Barbara; Jin, Zhijun; Freedman, Jonathan H.; Rubin, Charles S.

In: Journal of Biological Chemistry, Vol. 271, No. 47, 1996, p. 30158-30166.

Research output: Contribution to journalArticle

Linder, Barbara ; Jin, Zhijun ; Freedman, Jonathan H. ; Rubin, Charles S. / Molecular characterization of a novel, developmentally regulated small embryonic chaperone from Caenorhabditis elegans. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 47. pp. 30158-30166.
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