Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex

Paula Upla, Seung Joong Kim, Parthasarathy Sampathkumar, Kaushik Dutta, Sean M. Cahill, Ilan E. Chemmama, Rosemary Williams, Jeffrey B. Bonanno, William J. Rice, David L. Stokes, David Cowburn, Steven C. Almo, Andrej Sali, Michael P. Rout, Javier Fernandez-Martinez

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The membrane ring that equatorially circumscribes the nuclear pore complex (NPC) in the perinuclear lumen of the nuclear envelope is composed largely of Pom152 in yeast and its ortholog Nup210 (or Gp210) in vertebrates. Here, we have used a combination of negative-stain electron microscopy, nuclear magnetic resonance, and small-angle X-ray scattering methods to determine an integrative structure of the ∼120 kDa luminal domain of Pom152. Our structural analysis reveals that the luminal domain is formed by a flexible string-of-pearls arrangement of nine repetitive cadherin-like Ig-like domains, indicating an evolutionary connection between NPCs and the cell adhesion machinery. The 16 copies of Pom152 known to be present in the yeast NPC are long enough to form the observed membrane ring, suggesting how interactions between Pom152 molecules help establish and maintain the NPC architecture.

Original languageEnglish (US)
Pages (from-to)434-445
Number of pages12
JournalStructure
Volume25
Issue number3
DOIs
StatePublished - Mar 7 2017

Fingerprint

Nuclear Pore
Membranes
Yeasts
Nuclear Envelope
Cadherins
Cell Adhesion
Vertebrates
Electron Microscopy
Coloring Agents
Magnetic Resonance Spectroscopy
X-Rays

Keywords

  • cadherin
  • electron microscopy
  • Gp210
  • integrative structure determination
  • NMR
  • nuclear pore complex
  • nucleoporin
  • Nup210
  • Pom152
  • SAXS

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

Upla, P., Kim, S. J., Sampathkumar, P., Dutta, K., Cahill, S. M., Chemmama, I. E., ... Fernandez-Martinez, J. (2017). Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex. Structure, 25(3), 434-445. https://doi.org/10.1016/j.str.2017.01.006

Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex. / Upla, Paula; Kim, Seung Joong; Sampathkumar, Parthasarathy; Dutta, Kaushik; Cahill, Sean M.; Chemmama, Ilan E.; Williams, Rosemary; Bonanno, Jeffrey B.; Rice, William J.; Stokes, David L.; Cowburn, David; Almo, Steven C.; Sali, Andrej; Rout, Michael P.; Fernandez-Martinez, Javier.

In: Structure, Vol. 25, No. 3, 07.03.2017, p. 434-445.

Research output: Contribution to journalArticle

Upla, P, Kim, SJ, Sampathkumar, P, Dutta, K, Cahill, SM, Chemmama, IE, Williams, R, Bonanno, JB, Rice, WJ, Stokes, DL, Cowburn, D, Almo, SC, Sali, A, Rout, MP & Fernandez-Martinez, J 2017, 'Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex', Structure, vol. 25, no. 3, pp. 434-445. https://doi.org/10.1016/j.str.2017.01.006
Upla, Paula ; Kim, Seung Joong ; Sampathkumar, Parthasarathy ; Dutta, Kaushik ; Cahill, Sean M. ; Chemmama, Ilan E. ; Williams, Rosemary ; Bonanno, Jeffrey B. ; Rice, William J. ; Stokes, David L. ; Cowburn, David ; Almo, Steven C. ; Sali, Andrej ; Rout, Michael P. ; Fernandez-Martinez, Javier. / Molecular Architecture of the Major Membrane Ring Component of the Nuclear Pore Complex. In: Structure. 2017 ; Vol. 25, No. 3. pp. 434-445.
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