Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion

Jun Qiang Si, Zhi Qin Zhang, Chun Xia Li, Li Feng Wang, Yunlei Yang, Zhi Wang Li

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion (DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solution exchange. RESULTS: Application of SP (28/41, 68.5 %) and GABA (36/41, 88.2 %) could induce concentration-dependent inward current in some cells. SP-(10 μmol/L) and GABA (100 μmol/L)-activated inward currents were (244±83) pA (n=9) and (1.8±0.5) nA (n=13), respectively. The majority of GABA-activated current had obvious three processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP (0.001-1 μmol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peak value of GABA-activated current was more than the steady state of GABA-activated current. The inhibition of GABA-activated current by SP (0.1 μmol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8%±7.2%, n=7, P<0.01) and took about 12 min to get a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after blockade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitory effect on the peak value of GABA-activated current than the steady state of GABA-activated current.

Original languageEnglish (US)
Pages (from-to)623-629
Number of pages7
JournalActa Pharmacologica Sinica
Volume25
Issue number5
StatePublished - May 1 2004
Externally publishedYes

Fingerprint

Spinal Ganglia
Substance P
gamma-Aminobutyric Acid
Neurons
N-phthaloyl-4-aminobutyric acid
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Patch-Clamp Techniques
GABA-A Receptors

Keywords

  • GABA
  • Patch-clamp techniques
  • Protein kinase C
  • Spinal ganglia
  • Substance P

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Cite this

Si, J. Q., Zhang, Z. Q., Li, C. X., Wang, L. F., Yang, Y., & Li, Z. W. (2004). Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion. Acta Pharmacologica Sinica, 25(5), 623-629.

Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion. / Si, Jun Qiang; Zhang, Zhi Qin; Li, Chun Xia; Wang, Li Feng; Yang, Yunlei; Li, Zhi Wang.

In: Acta Pharmacologica Sinica, Vol. 25, No. 5, 01.05.2004, p. 623-629.

Research output: Contribution to journalArticle

Si, JQ, Zhang, ZQ, Li, CX, Wang, LF, Yang, Y & Li, ZW 2004, 'Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion', Acta Pharmacologica Sinica, vol. 25, no. 5, pp. 623-629.
Si, Jun Qiang ; Zhang, Zhi Qin ; Li, Chun Xia ; Wang, Li Feng ; Yang, Yunlei ; Li, Zhi Wang. / Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion. In: Acta Pharmacologica Sinica. 2004 ; Vol. 25, No. 5. pp. 623-629.
@article{b32ffd95d5e342f7ad4333b04d8dab68,
title = "Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion",
abstract = "AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion (DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solution exchange. RESULTS: Application of SP (28/41, 68.5 {\%}) and GABA (36/41, 88.2 {\%}) could induce concentration-dependent inward current in some cells. SP-(10 μmol/L) and GABA (100 μmol/L)-activated inward currents were (244±83) pA (n=9) and (1.8±0.5) nA (n=13), respectively. The majority of GABA-activated current had obvious three processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP (0.001-1 μmol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peak value of GABA-activated current was more than the steady state of GABA-activated current. The inhibition of GABA-activated current by SP (0.1 μmol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8{\%}±7.2{\%}, n=7, P<0.01) and took about 12 min to get a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after blockade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitory effect on the peak value of GABA-activated current than the steady state of GABA-activated current.",
keywords = "GABA, Patch-clamp techniques, Protein kinase C, Spinal ganglia, Substance P",
author = "Si, {Jun Qiang} and Zhang, {Zhi Qin} and Li, {Chun Xia} and Wang, {Li Feng} and Yunlei Yang and Li, {Zhi Wang}",
year = "2004",
month = "5",
day = "1",
language = "English (US)",
volume = "25",
pages = "623--629",
journal = "Acta Pharmacologica Sinica",
issn = "1671-4083",
publisher = "Nature Publishing Group",
number = "5",

}

TY - JOUR

T1 - Modulatory effect of substance P on GABA-activated currents from rat dorsal root ganglion

AU - Si, Jun Qiang

AU - Zhang, Zhi Qin

AU - Li, Chun Xia

AU - Wang, Li Feng

AU - Yang, Yunlei

AU - Li, Zhi Wang

PY - 2004/5/1

Y1 - 2004/5/1

N2 - AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion (DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solution exchange. RESULTS: Application of SP (28/41, 68.5 %) and GABA (36/41, 88.2 %) could induce concentration-dependent inward current in some cells. SP-(10 μmol/L) and GABA (100 μmol/L)-activated inward currents were (244±83) pA (n=9) and (1.8±0.5) nA (n=13), respectively. The majority of GABA-activated current had obvious three processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP (0.001-1 μmol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peak value of GABA-activated current was more than the steady state of GABA-activated current. The inhibition of GABA-activated current by SP (0.1 μmol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8%±7.2%, n=7, P<0.01) and took about 12 min to get a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after blockade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitory effect on the peak value of GABA-activated current than the steady state of GABA-activated current.

AB - AIM: To explore the modulatory effect of substance P (SP) on GABA-activated current of dorsal root ganglion (DRG) neurons in rat. METHODS: The whole-cell patch-clamp technique was used to record SP- and GABA-activated currents in neurons freshly dissociated from rat DRG neurons. Drugs were applied by rapid solution exchange. RESULTS: Application of SP (28/41, 68.5 %) and GABA (36/41, 88.2 %) could induce concentration-dependent inward current in some cells. SP-(10 μmol/L) and GABA (100 μmol/L)-activated inward currents were (244±83) pA (n=9) and (1.8±0.5) nA (n=13), respectively. The majority of GABA-activated current had obvious three processes, the peak value (Ip), the steady state (Iss) and the desensitization (Id). The desensitization of GABA-activated current was a biphasic process, including fast and slow desensitization. However, pre-application of SP (0.001-1 μmol/L) could inhibit the GABA-activated inward current which was identified to be GABAA receptor-mediated current. The inhibitory effects were concentration-dependent. The inhibitory effect of SP on the peak value of GABA-activated current was more than the steady state of GABA-activated current. The inhibition of GABA-activated current by SP (0.1 μmol/L) was related to the time after application of SP, the inhibition of GABA-activated currents by SP reached the peak at about 4 min (49.8%±7.2%, n=7, P<0.01) and took about 12 min to get a full recovery. The inhibition of GABA-activated currents by SP was almost completely removed after blockade of PKC by H-7 with the re-patch clamp. CONCLUSION: Pre-application of SP exerts a more strong inhibitory effect on the peak value of GABA-activated current than the steady state of GABA-activated current.

KW - GABA

KW - Patch-clamp techniques

KW - Protein kinase C

KW - Spinal ganglia

KW - Substance P

UR - http://www.scopus.com/inward/record.url?scp=2442497219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2442497219&partnerID=8YFLogxK

M3 - Article

VL - 25

SP - 623

EP - 629

JO - Acta Pharmacologica Sinica

JF - Acta Pharmacologica Sinica

SN - 1671-4083

IS - 5

ER -