Modulation of tumor-host interactions, angiogenesis, and tumor growth by tissue inhibitor of metalloproteinase 2 via a novel mechanism

Andrew L. Feldman, William G. Stetler-Stevenson, Nick G. Costouros, Vladimir Knezevic, Galina Baibakov, H. Richard Alexander, Dominique Lorang, Stephen M. Hewitt, Dong Wan Seo, Marshall S. Miller, Sarah O'Connor, Steven K. Libutti

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Solid tumors depend on angiogenesis for sustained growth. Tissue inhibitor of metalloproteinase 2 (TIMP-2) is an angiogenesis inhibitor initially characterized for its ability to block matrix metalloproteinases; however, recent data suggest that the antiangiogenic action of TIMP-2 may rely on matrix metalloproteinase-independent mechanisms. The aim of this study was to identify molecular pathways involved in the effects of TIMP-2 on processes dependent on tumor-host interactions such as angiogenesis. Using in vitro cell culture and a syngeneic murine tumor model, we compared the effects of TIMP-2 overexpression on gene expression profiles in vitro to those observed in vivo. Validating these findings by real-time quantitative PCR and layered protein scanning, we identified up-regulation of mitogen-activated protein kinase phosphatase 1 as an effector of the antiangiogenic function of TIMP-2. Up-regulation of mitogen-activated protein kinase phosphatase 1 in tumors overexpressing TIMP-2 leads to dephosphorylation of p38 mitogen-activated protein kinase and inhibition of tumor growth and angiogenesis. Phosphatase activity appears important in regulating tumor angiogenesis, offering a promising direction for the identification of novel molecular targets and antiangiogenic compounds for the treatment of cancer.

Original languageEnglish (US)
Pages (from-to)4481-4486
Number of pages6
JournalCancer Research
Volume64
Issue number13
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

Fingerprint

Tissue Inhibitor of Metalloproteinase-2
Growth Inhibitors
Mitogen-Activated Protein Kinase Phosphatases
Dual Specificity Phosphatase 1
Neoplasms
Matrix Metalloproteinases
Up-Regulation
Angiogenesis Inhibitors
p38 Mitogen-Activated Protein Kinases
Growth
Transcriptome
Phosphoric Monoester Hydrolases
Real-Time Polymerase Chain Reaction
Cell Culture Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Feldman, A. L., Stetler-Stevenson, W. G., Costouros, N. G., Knezevic, V., Baibakov, G., Alexander, H. R., ... Libutti, S. K. (2004). Modulation of tumor-host interactions, angiogenesis, and tumor growth by tissue inhibitor of metalloproteinase 2 via a novel mechanism. Cancer Research, 64(13), 4481-4486. https://doi.org/10.1158/0008-5472.CAN-03-2929

Modulation of tumor-host interactions, angiogenesis, and tumor growth by tissue inhibitor of metalloproteinase 2 via a novel mechanism. / Feldman, Andrew L.; Stetler-Stevenson, William G.; Costouros, Nick G.; Knezevic, Vladimir; Baibakov, Galina; Alexander, H. Richard; Lorang, Dominique; Hewitt, Stephen M.; Seo, Dong Wan; Miller, Marshall S.; O'Connor, Sarah; Libutti, Steven K.

In: Cancer Research, Vol. 64, No. 13, 01.07.2004, p. 4481-4486.

Research output: Contribution to journalArticle

Feldman, AL, Stetler-Stevenson, WG, Costouros, NG, Knezevic, V, Baibakov, G, Alexander, HR, Lorang, D, Hewitt, SM, Seo, DW, Miller, MS, O'Connor, S & Libutti, SK 2004, 'Modulation of tumor-host interactions, angiogenesis, and tumor growth by tissue inhibitor of metalloproteinase 2 via a novel mechanism', Cancer Research, vol. 64, no. 13, pp. 4481-4486. https://doi.org/10.1158/0008-5472.CAN-03-2929
Feldman, Andrew L. ; Stetler-Stevenson, William G. ; Costouros, Nick G. ; Knezevic, Vladimir ; Baibakov, Galina ; Alexander, H. Richard ; Lorang, Dominique ; Hewitt, Stephen M. ; Seo, Dong Wan ; Miller, Marshall S. ; O'Connor, Sarah ; Libutti, Steven K. / Modulation of tumor-host interactions, angiogenesis, and tumor growth by tissue inhibitor of metalloproteinase 2 via a novel mechanism. In: Cancer Research. 2004 ; Vol. 64, No. 13. pp. 4481-4486.
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