Modulation of phagosome-lysosome fusion in mouse macrophages

M. C. Kielian, Z. A. Cohn

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

A previously described fluorescence assay has been used to characterize factors that modulate phagosome-lysosome (P-L) fusion in mouse macrophages. Fusion was not affected by enzymatic modification or by concanavalin A cross-linking of the plasma membrane or by coating the phagocytic particle with concanavalin A or immune serum. Pretreatment of cells with 10-5-10-4M colchicine, or treatment immediately after ingestion with 1-10μg/ml cytochalasin did not alter P-L fusion; implying that the cytoskeleton does not control fusion in a rate-limiting way. Fusion was strikingly elevated in 5 hr cultures of activated macrophages from immune-boosted mice. A lower enhancement was seen in cells activated by proteose-peptone, a nonspecific inflammatory agent.

Original languageEnglish (US)
Pages (from-to)1015-1020
Number of pages6
JournalJournal of Experimental Medicine
Volume153
Issue number4
DOIs
Publication statusPublished - Jan 1 1981
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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