Modulation of LINE-1 retrotransposition by a human SAMHD1 polymorphism

Tommy E. White, Alberto Brandariz-Nuñez, Kyudong Han, Sara L. Sawyer, Baek Kim, Felipe Diaz-Griffero

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The HIV-1 restriction factor SAMHD1 has the ability to negatively modulate retrotransposition of the long interspersed element 1(LINE-1). By exploring the ability of human SAMHD1 polymorphisms to inhibit LINE-1, we found that the single nucleotide polymorphism S33A present in the Korean population lose the ability to inhibit LINE-1 retrotransposition. Because SAMHD1 residue S33 is phosphorylated in human cycling and non-cycling cells, we demonstrated that SAMHD1 requires to be either phosphorylated on position 33 or to contain a bulky residue in order to inhibit LINE-1 retrotransposition. Therefore this unique mutation uncouples functions in this important restriction factor.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalVirology Reports
Volume6
DOIs
Publication statusPublished - Dec 1 2016

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Keywords

  • HIV-1
  • LINE-1
  • Restriction
  • Retrotransposition
  • SAMHD1
  • SNPs

ASJC Scopus subject areas

  • Virology
  • Biochemistry

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