TY - JOUR
T1 - Modulation of K+ channel currents by serum amineoxidase in neurons
AU - Wu, Lingyun
AU - Mateescu, Mircea Alexandru
AU - Wang, Xin Tao
AU - Mondovi, Bruno
AU - Wang, Rui
N1 - Funding Information:
This work was supported by a joint MRC (Canada)–CNR (Italy) research agreement (M.A.M and B.M) and by grants from Medical Research Council (MRC), from CNR project on “Biotecnologie e Biologia Moleculare” and from MURST (Italy). R. Wang is a McDonald Scholar of the Heart and Stroke Foundation of Canada.
PY - 1996/3/7
Y1 - 1996/3/7
N2 - The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.
AB - The influence of bovine serum amineoxidase (SAO), a circulating copper-enzyme, on neuronal K+ channels is described. Bovine SAO enhanced K+ channel currents in N1E-115 neuroblastoma cells in a time-dependent manner. Unlike ceruloplasmin (another copper-protein, shown as depolarizing factor in neurons), SAO had no effect on resting potential of neurons. However, pretreatment of cells with SAO inhibited ceruloplasmin-induced membrane depolarization. Although ceruloplasmin alone inhibited K+ channel currents, it further enhanced K+ channel currents in the presence of SAO. Therefore, SAO may be another endogenous modulator of neuronal K+ channels with effect and mechanisms different from those of ceruloplasmin.
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U2 - 10.1006/bbrc.1996.0354
DO - 10.1006/bbrc.1996.0354
M3 - Article
C2 - 8602855
AN - SCOPUS:0030009239
SN - 0006-291X
VL - 220
SP - 47
EP - 52
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -