TY - JOUR
T1 - Modulation of humoral immune responses in the rat by centrally applied Met-Enk and opioid receptor antagonists
T2 - Functional interactions of brain OP1, OP2 and OP3 receptors
AU - Dimitrijevi, Mirjana
AU - Stanojevi, Stanislava
AU - Kovacevi-Jovanovi, Vesna
AU - Mileti, Tatjana
AU - Vuji-Redzi, Vesna
AU - Radulovi, Jelena
N1 - Funding Information:
The technical assistance of Mrs. Zuzana Tomaš is gratefully acknowledged. This work was supported by the Ministry of Science and Technology of the Republic of Serbia, Belgrade, Yugoslavia.
PY - 2000
Y1 - 2000
N2 - We have previously demonstrated that central application of leucine- enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (δ) and OP2 (κ) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (μ) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met- Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor- selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor- binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist β-funaltrexamine (β-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by β-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met- Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V.
AB - We have previously demonstrated that central application of leucine- enkephalin (Leu-Enk) elicits potentiation and suppression of humoral immune responses through OP1 (δ) and OP2 (κ) receptors, respectively. Interestingly, both effects were found to be additionally dependent on OP3 (μ) receptor function. In the present study, we have further investigated whether opioid receptor interactions underlie the immunomodulatory effects of endogenous opioids as well as exogenously applied methionine-enkephalin (Met- Enk). For that purpose, the plaque-forming cell (PFC) response was determined in rats injected intracerebroventricularly (i.c.v.) with opioid receptor- selective antagonists and Met-Enk. Application of the OP1 antagonist ICI 174864, but not naltrindole, resulted in suppression of the PFC response. In contrast, i.c.v. injection of the OP2 selective antagonist nor- binaltorphimine (nor-BNI) significantly potentiated the PFC response. Both effects, presumably mediated by endogenous opioid peptides, were antagonized by the OP3 receptor antagonist β-funaltrexamine (β-FNA) at a dose that was devoid of immunomodulatory activity. The immunopotentiation of the PFC response induced by Met-Enk was reversed by OP1 receptor antagonists, naltrindole and ICI 174864, but not by β-FNA or nor-BNI. On the basis of these and previous findings, it may be concluded that central OP3 receptors are permissive for the central immunomodulatory action of endogenous opioid peptides and Leu-Enk. In contrast, the central immunoenhancing effect of Met- Enk appears to be mediated through OP3-independent OP1 receptors. (C) 2000 Elsevier Science B.V.
KW - Central nervous system
KW - Leucine-enkephalin
KW - Methionine-enkephalin
KW - OP
KW - OP
KW - OP
KW - Opioid receptors
KW - Plaque-forming cell response
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U2 - 10.1016/S0162-3109(00)00213-7
DO - 10.1016/S0162-3109(00)00213-7
M3 - Article
C2 - 10996023
AN - SCOPUS:0033829010
SN - 0162-3109
VL - 49
SP - 255
EP - 262
JO - Immunopharmacology
JF - Immunopharmacology
IS - 3
ER -