TY - JOUR
T1 - Modulation of humoral immune response by central administration of leucine-enkephalin
T2 - Effects of μ, ∂ and κ opioid receptor antagonists
AU - Radulović, Jelena
AU - Mančev, Zorica
AU - Stanojević, Stanislava
AU - Vasiljević, Tatjana
AU - Kovačević-Jovanović, Vesna
AU - Pešić, Gordana
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1996/4
Y1 - 1996/4
N2 - The effect of leucine-enkephalin (Leu-Enk) on primary humoral immune response was investigated following intracerebroventricular (i.c.v.) administration of the peptide in the rat. Leu-Enk stimulated plaque-forming cell (PFC) response in rats i.c.v. injected with 0.1 and 1 μg/kg, whereas doses of 20 and 50 μg/kg exerted immunosuppressive effects. I.c.v. treatment of rats with ∂ opioid receptor antagonist ICI 174864 and κ opioid receptor antagonist nor-binaltorphimine (nor-BNI) blocked stimulation and suppression of PFC response induced by Leu-Enk, respectively. The μ opioid receptor antagonist β-funaltrexamine (β-FNA) reversed both immunomodulatory effects produced by Leu-Enk. Since β-FNA alone had no effect on PFC response (unlike ICI 174 864 and nor-BNI), these data showed that central effects of Leu-Enk on PFC response were mediated by brain μ opioid receptors, and suggested a possible involvement of ∂ and κ opioid receptors.
AB - The effect of leucine-enkephalin (Leu-Enk) on primary humoral immune response was investigated following intracerebroventricular (i.c.v.) administration of the peptide in the rat. Leu-Enk stimulated plaque-forming cell (PFC) response in rats i.c.v. injected with 0.1 and 1 μg/kg, whereas doses of 20 and 50 μg/kg exerted immunosuppressive effects. I.c.v. treatment of rats with ∂ opioid receptor antagonist ICI 174864 and κ opioid receptor antagonist nor-binaltorphimine (nor-BNI) blocked stimulation and suppression of PFC response induced by Leu-Enk, respectively. The μ opioid receptor antagonist β-funaltrexamine (β-FNA) reversed both immunomodulatory effects produced by Leu-Enk. Since β-FNA alone had no effect on PFC response (unlike ICI 174 864 and nor-BNI), these data showed that central effects of Leu-Enk on PFC response were mediated by brain μ opioid receptors, and suggested a possible involvement of ∂ and κ opioid receptors.
KW - Central nervous system
KW - Leucine-enkephalin
KW - Plaque-forming cell response
KW - κ opioid receptors
KW - μ opioid receptors
KW - ∂ opioid receptors
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U2 - 10.1016/0165-5728(96)00017-3
DO - 10.1016/0165-5728(96)00017-3
M3 - Article
C2 - 8964897
AN - SCOPUS:0029890541
SN - 0165-5728
VL - 65
SP - 155
EP - 161
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 2
ER -