Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

Outhiriaradjou Benard, K. A. Balasubramanian

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.

Original languageEnglish (US)
Pages (from-to)109-114
Number of pages6
JournalMolecular and Cellular Biochemistry
Volume170
Issue number1-2
DOIs
StatePublished - May 12 1997
Externally publishedYes

Keywords

  • BSO
  • Butyrate
  • Differentiation
  • GSH
  • HT-29 cells
  • Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells'. Together they form a unique fingerprint.

  • Cite this