Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

Outhiriaradjou Benard; K. A. Balasubramanian

doi:10.1023/A:1006892929652

Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

Outhiriaradjou Benard, K. A. Balasubramanian

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.

Original language	English (US)
Pages (from-to)	109-114
Number of pages	6
Journal	Molecular and Cellular Biochemistry
Volume	170
Issue number	1-2
DOIs	https://doi.org/10.1023/A:1006892929652
State	Published - 1997
Externally published	Yes

Keywords

BSO
Butyrate
Differentiation
GSH
HT-29 cells
Proliferation

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1023/A:1006892929652

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title = "Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells",

abstract = "Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.",

keywords = "BSO, Butyrate, Differentiation, GSH, HT-29 cells, Proliferation",

author = "Outhiriaradjou Benard and Balasubramanian, {K. A.}",

note = "Funding Information: The Wellcome Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Council of Scientific and Industrial Research and Indian Council of Medical Research, Government of India is acknowledged. The authors thank Professor V.I. Mathan for his keen interest in this work. Outhiriaradjou Benard is a Senior Research Fellow of the Council of Scientific and Industrial Research, India. Authors thank Mr. G. Subramani for his help in cell culture work.",

year = "1997",

doi = "10.1023/A:1006892929652",

language = "English (US)",

volume = "170",

pages = "109--114",

journal = "Molecular and Cellular Biochemistry",

issn = "0300-8177",

publisher = "Springer Netherlands",

number = "1-2",

}

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T1 - Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

AU - Benard, Outhiriaradjou

AU - Balasubramanian, K. A.

N1 - Funding Information: The Wellcome Research Laboratory is supported by The Wellcome Trust, London. Financial assistance from the Council of Scientific and Industrial Research and Indian Council of Medical Research, Government of India is acknowledged. The authors thank Professor V.I. Mathan for his keen interest in this work. Outhiriaradjou Benard is a Senior Research Fellow of the Council of Scientific and Industrial Research, India. Authors thank Mr. G. Subramani for his help in cell culture work.

PY - 1997

Y1 - 1997

N2 - Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.

AB - Glutathione plays an important role in various cellular functions including cell growth and differentiation. In the present study, cell differentiation was induced by butyrate in human colon cell line HT-29 and cellular thiol status was assessed. It was observed that butyrate-induced differentiation was associated with decrease in cellular GSH level and this was prominent at early stages of differentiation. Buthionine sulfoximine (BSO), a specific cellular GSH depleting agent, did not induce differentiation in cells but potentiated the differentiation induced by butyrate. Both BSO and butyrate individually and together inhibited cell growth. These studies suggest that cellular GSH level is modulated in butyrate-induced differentiation and decrease of GSH at the initial stage might facilitate cellular differentiation.

KW - BSO

KW - Butyrate

KW - Differentiation

KW - GSH

KW - HT-29 cells

KW - Proliferation

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JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

IS - 1-2

ER -

Modulation of glutathione level during butyrate-induced differentiation in human colon derived HT-29 cells

Abstract

Keywords

ASJC Scopus subject areas

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