Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates

Hua Wang, Yi He, Buddy D. Ratner, Shaoyi Jiang

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In this work, we demonstrate that surface charge can be used to modulate cell adhesion/spreading through the control of the orientation of adsorbed FnIII7-10, which is a cell-adhesive protein containing RGD residues. Carboxylic acid (COOH) and amine (NH2)-terminated self-assembled monolayers (SAMs) of alkanethiolates were used as model negatively and positively charged surfaces, respectively. The adsorbed amount of FnIII 7-10 is controlled to be equivalent on both SAMs as confirmed by the adsorption isotherms determined using I125-radiolabeled FnIII 7-10 The binding of a monoclonal antibody specific for the cell-binding domain of FnIII7-10 was measured by surface plasmon resonance (SPR) to evaluate FnIII7-10 orientations on different SAMs. Results indicate that adsorbed FnIII7-10 on NH2-SAM has an orientation with more cell-binding domains accessible than on COOH-SAM, confirming our predictions from Monte Carlo simulations. Both phase contrast images and Vybrant® MTT cell proliferation assays show that the adhesion/spreading of bovine aortic endothelial cells (BAECs) on the NH 2-SAM is significantly better than that on the COOH-SAM coated with an equivalent amount of FnIII7-10. These results indicate that surface charge can be used to specifically orient cell adhesive proteins such as FnIII7-10, thus providing a promising strategy to increase the activity of materials incorporating biological moieties.

Original languageEnglish (US)
Pages (from-to)672-678
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume77
Issue number4
DOIs
StatePublished - Jun 15 2006
Externally publishedYes

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Cell adhesion
Self assembled monolayers
Surface charge
Adhesives
Proteins
Monoclonal antibodies
Endothelial cells
Cell proliferation
Surface plasmon resonance
Carboxylic Acids
Carboxylic acids
Adsorption isotherms
Biological materials
Amines
Assays
Adhesion
Monoclonal Antibodies

Keywords

  • Biomaterials
  • Cell adhesion and spreading
  • FnIII
  • Protein orientation
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Modulating cell adhesion and spreading by control of FnIII7-10 orientation on charged self-assembled monolayers (SAMs) of alkanethiolates. / Wang, Hua; He, Yi; Ratner, Buddy D.; Jiang, Shaoyi.

In: Journal of Biomedical Materials Research - Part A, Vol. 77, No. 4, 15.06.2006, p. 672-678.

Research output: Contribution to journalArticle

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