TY - JOUR
T1 - Modular scanning confocal microscope with digital image processing
AU - Ye, Xianjun
AU - McCluskey, Matthew D.
N1 - Publisher Copyright:
© 2016 Ye, McCluskey. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/11/1
Y1 - 2016/11/1
N2 - In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip carrier, and a piece of etched silicon wafer. The data collected by the CCD were processed to yield images of the specimen. By selecting effective pixels in the recorded CCD images, a virtual pinhole is created. By analyzing the image moments of the imaging data, a lateral resolution enhancement is achieved by using a 20 x / NA = 0.4 microscope objective at 532 nm laser wavelength.
AB - In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip carrier, and a piece of etched silicon wafer. The data collected by the CCD were processed to yield images of the specimen. By selecting effective pixels in the recorded CCD images, a virtual pinhole is created. By analyzing the image moments of the imaging data, a lateral resolution enhancement is achieved by using a 20 x / NA = 0.4 microscope objective at 532 nm laser wavelength.
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U2 - 10.1371/journal.pone.0166212
DO - 10.1371/journal.pone.0166212
M3 - Article
C2 - 27829052
AN - SCOPUS:84994475182
SN - 1932-6203
VL - 11
JO - PloS one
JF - PloS one
IS - 11
M1 - e0166212
ER -