Modular scanning confocal microscope with digital image processing

Xianjun Ye, Matthew D. McCluskey

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In conventional confocal microscopy, a physical pinhole is placed at the image plane prior to the detector to limit the observation volume. In this work, we present a modular design of a scanning confocal microscope which uses a CCD camera to replace the physical pinhole for materials science applications. Experimental scans were performed on a microscope resolution target, a semiconductor chip carrier, and a piece of etched silicon wafer. The data collected by the CCD were processed to yield images of the specimen. By selecting effective pixels in the recorded CCD images, a virtual pinhole is created. By analyzing the image moments of the imaging data, a lateral resolution enhancement is achieved by using a 20 x / NA = 0.4 microscope objective at 532 nm laser wavelength.

Original languageEnglish (US)
Article numbere0166212
JournalPloS one
Volume11
Issue number11
DOIs
StatePublished - Nov 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint

Dive into the research topics of 'Modular scanning confocal microscope with digital image processing'. Together they form a unique fingerprint.

Cite this