Mode‐specific relaxation dynamics of photoexcited fe(II) protoporphyrin IX in hemoglobin

R. G. Alden, M. C. Schneebeck, M. R. Ondrias, S. H. Courtney, Joel M. Friedman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The behavior of the resonance Raman spectra of deoxyhemoglobin in the presence of strong resonant fields was studied by using ca. 30 ps laser pulses. The line width of the strongest Raman‐active mode in the Soret absorption band region, v4, broadens as a function of laser fluence, and its anti‐Stokes/Stokes intensity ratio is power dependent. Previous studies have suggested that equipartition of excess vibrational energy occurs on very fast time scales in the heme macrocycle subsequent to absorption of a photon. The mode‐specific vibrational dynamics of v4, however, indicate that the vibrational relaxation of the chromophore cannot be treated within the context of an equipartition of energy, but rather should be evaluated in terms of the relaxation phenomena of the specific vibrational mode. These data are evaluated in terms of relaxation mechanisms of the vibrational mode with reference to power‐broadening processes and transient vibrational population redistribution.

Original languageEnglish (US)
Pages (from-to)569-574
Number of pages6
JournalJournal of Raman Spectroscopy
Volume23
Issue number10
DOIs
StatePublished - 1992

Fingerprint

Hemoglobin
Chromophores
Heme
Linewidth
Raman scattering
Absorption spectra
Laser pulses
Hemoglobins
Photons
Lasers
deoxyhemoglobin
protoporphyrin IX

ASJC Scopus subject areas

  • Materials Science(all)
  • Spectroscopy

Cite this

Mode‐specific relaxation dynamics of photoexcited fe(II) protoporphyrin IX in hemoglobin. / Alden, R. G.; Schneebeck, M. C.; Ondrias, M. R.; Courtney, S. H.; Friedman, Joel M.

In: Journal of Raman Spectroscopy, Vol. 23, No. 10, 1992, p. 569-574.

Research output: Contribution to journalArticle

Alden, R. G. ; Schneebeck, M. C. ; Ondrias, M. R. ; Courtney, S. H. ; Friedman, Joel M. / Mode‐specific relaxation dynamics of photoexcited fe(II) protoporphyrin IX in hemoglobin. In: Journal of Raman Spectroscopy. 1992 ; Vol. 23, No. 10. pp. 569-574.
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