Modern fluorescent proteins: From chromophore formation to novel intracellular applications

Olesya V. Stepanenko, Olga V. Stepanenko, Daria Shcherbakova, Irina M. Kuznetsova, Konstantin K. Turoverov, Vladislav Verkhusha

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.

Original languageEnglish (US)
Pages (from-to)313-327
Number of pages15
JournalBioTechniques
Volume51
Issue number5
DOIs
StatePublished - Nov 2011

Fingerprint

Chromophores
Proteins
Imaging techniques
Microscopy
Microscopic examination
Protein Engineering
Photons
Color
Animals
Modulation
Tissue
Molecules

Keywords

  • FRET
  • mKate
  • PALM
  • PAmcherry
  • STED
  • STORM
  • Two-photon microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biotechnology

Cite this

Modern fluorescent proteins : From chromophore formation to novel intracellular applications. / Stepanenko, Olesya V.; Stepanenko, Olga V.; Shcherbakova, Daria; Kuznetsova, Irina M.; Turoverov, Konstantin K.; Verkhusha, Vladislav.

In: BioTechniques, Vol. 51, No. 5, 11.2011, p. 313-327.

Research output: Contribution to journalArticle

Stepanenko, Olesya V. ; Stepanenko, Olga V. ; Shcherbakova, Daria ; Kuznetsova, Irina M. ; Turoverov, Konstantin K. ; Verkhusha, Vladislav. / Modern fluorescent proteins : From chromophore formation to novel intracellular applications. In: BioTechniques. 2011 ; Vol. 51, No. 5. pp. 313-327.
@article{0483986087b84ba6bfd232055d6021ae,
title = "Modern fluorescent proteins: From chromophore formation to novel intracellular applications",
abstract = "The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.",
keywords = "FRET, mKate, PALM, PAmcherry, STED, STORM, Two-photon microscopy",
author = "Stepanenko, {Olesya V.} and Stepanenko, {Olga V.} and Daria Shcherbakova and Kuznetsova, {Irina M.} and Turoverov, {Konstantin K.} and Vladislav Verkhusha",
year = "2011",
month = "11",
doi = "10.2144/000113765",
language = "English (US)",
volume = "51",
pages = "313--327",
journal = "BioTechniques",
issn = "0736-6205",
publisher = "Eaton Publishing Company",
number = "5",

}

TY - JOUR

T1 - Modern fluorescent proteins

T2 - From chromophore formation to novel intracellular applications

AU - Stepanenko, Olesya V.

AU - Stepanenko, Olga V.

AU - Shcherbakova, Daria

AU - Kuznetsova, Irina M.

AU - Turoverov, Konstantin K.

AU - Verkhusha, Vladislav

PY - 2011/11

Y1 - 2011/11

N2 - The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.

AB - The diverse biochemical and photophysical properties of fluorescent proteins (FPs) have enabled the generation of a growing palette of colors, providing unique opportunities for their use in a variety of modern biology applications. Modulation of these FP characteristics is achieved through diversity in both the structure of the chromophore as well as the contacts between the chromophore and the surrounding protein barrel. Here we review our current knowledge of blue, green, and red chromophore formation in permanently emitting FPs, photoactivatable FPs, and fluorescent timers. Progress in understanding the interplay between FP structure and function has allowed the engineering of FPs with many desirable features, and enabled recent advances in microscopy techniques such as super-resolution imaging of single molecules, imaging of protein dynamics, photochromic FRET, deep-tissue imaging, and multicolor two-photon microscopy in live animals.

KW - FRET

KW - mKate

KW - PALM

KW - PAmcherry

KW - STED

KW - STORM

KW - Two-photon microscopy

UR - http://www.scopus.com/inward/record.url?scp=80655139792&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80655139792&partnerID=8YFLogxK

U2 - 10.2144/000113765

DO - 10.2144/000113765

M3 - Article

C2 - 22054544

AN - SCOPUS:80655139792

VL - 51

SP - 313

EP - 327

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 5

ER -