Modern fluorescent proteins and imaging technologies to study gene expression, nuclear localization, and dynamics

Bin Wu, Kiryl D. Piatkevich, Timothée Lionnet, Robert H. Singer, Vladislav V. Verkhusha

Research output: Contribution to journalReview articlepeer-review

105 Scopus citations

Abstract

Recent developments in reagent design can address problems in single cells that were not previously approachable. We have attempted to foresee what will become possible, and the sorts of biological problems that become tractable with these novel reagents. We have focused on the novel fluorescent proteins that allow convenient multiplexing, and provide for a time-dependent analysis of events in single cells. Methods for fluorescently labeling specific molecules, including endogenously expressed proteins and mRNA have progressed and are now commonly used in a variety of organisms. Finally, sensitive microscopic methods have become more routine practice. This article emphasizes that the time is right to coordinate these approaches for a new initiative on single cell imaging of biological molecules.

Original languageEnglish (US)
Pages (from-to)310-317
Number of pages8
JournalCurrent Opinion in Cell Biology
Volume23
Issue number3
DOIs
StatePublished - Jun 1 2011

ASJC Scopus subject areas

  • Cell Biology

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