Modern Electron Microscopy Methods for C. elegans

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

From its inception as a model organism 40 years ago, Caenorhabditis elegans was chosen in part for its suitability for study in serial thin sections by electron microscopy. Recent improvements in electron microscopy technology are making this pursuit more reliable and more powerful. In this chapter, we highlight new methods in specimen preparation, imaging, and data analysis. Accurate three-dimensional information can now be obtained for the whole animal at all stages, down to the level of individual organelles and the cytoskeleton.

Original languageEnglish (US)
Pages (from-to)93-149
Number of pages57
JournalMethods in Cell Biology
Volume107
DOIs
StatePublished - 2012

Fingerprint

Electron Microscopy
Caenorhabditis elegans
Cytoskeleton
Organelles
Technology

Keywords

  • Fixation
  • Immersion
  • Immunocytochemistry
  • Reconstruction
  • Sectioning
  • Tomography

ASJC Scopus subject areas

  • Cell Biology

Cite this

Modern Electron Microscopy Methods for C. elegans. / Hall, David H.; Hartwieg, Erika; Nguyen, Ken C.Q.

In: Methods in Cell Biology, Vol. 107, 2012, p. 93-149.

Research output: Contribution to journalArticle

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