@inbook{c6794f1b5fde4338adb4bbd03ccf8a75,
title = "Modern Electron Microscopy Methods for C. elegans",
abstract = "From its inception as a model organism 40 years ago, Caenorhabditis elegans was chosen in part for its suitability for study in serial thin sections by electron microscopy. Recent improvements in electron microscopy technology are making this pursuit more reliable and more powerful. In this chapter, we highlight new methods in specimen preparation, imaging, and data analysis. Accurate three-dimensional information can now be obtained for the whole animal at all stages, down to the level of individual organelles and the cytoskeleton.",
keywords = "Fixation, Immersion, Immunocytochemistry, Reconstruction, Sectioning, Tomography",
author = "Hall, {David H.} and Erika Hartwieg and Nguyen, {Ken C.Q.}",
note = "Funding Information: Detailed TEM protocols were developed in the Hall lab by Ken Nguyen, or in the Horvitz lab by Erika Hartwieg. We have also enjoyed help from many sources. In the Hall lab, technical help has come from Gloria Stephney, Maguy Paupard, Leslie Gunther, Christine Roy, Kevin Fisher, and a series of talented students and visitors, especially Agnessa Miller, Irina Kolotuev and Kristin Politi. Work in the Hall lab is supported by NIH RR 12596. Erika Hartwieg's work was supported by HHMI to Bob Horvitz. ",
year = "2012",
doi = "10.1016/B978-0-12-394620-1.00004-7",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "93--149",
booktitle = "Methods in Cell Biology",
}