Modern Electron Microscopy Methods for C. elegans

Research output: Chapter in Book/Report/Conference proceedingChapter

51 Scopus citations

Abstract

From its inception as a model organism 40 years ago, Caenorhabditis elegans was chosen in part for its suitability for study in serial thin sections by electron microscopy. Recent improvements in electron microscopy technology are making this pursuit more reliable and more powerful. In this chapter, we highlight new methods in specimen preparation, imaging, and data analysis. Accurate three-dimensional information can now be obtained for the whole animal at all stages, down to the level of individual organelles and the cytoskeleton.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages93-149
Number of pages57
DOIs
StatePublished - 2012

Publication series

NameMethods in Cell Biology
Volume107
ISSN (Print)0091-679X

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Keywords

  • Fixation
  • Immersion
  • Immunocytochemistry
  • Reconstruction
  • Sectioning
  • Tomography

ASJC Scopus subject areas

  • Cell Biology

Cite this

Hall, D. H., Hartwieg, E., & Nguyen, K. C. Q. (2012). Modern Electron Microscopy Methods for C. elegans. In Methods in Cell Biology (pp. 93-149). (Methods in Cell Biology; Vol. 107). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-394620-1.00004-7